Clostridium difficile (Hall and O'Toole) Prevot 拉丁名
(ATCC? BAA-1382-FZ?) 統(tǒng)一編號(hào)
Strain Designations 630
Isolation 分離基物 Clinical isolate Switzerland
Biosafety Level 生物安全等級(jí) 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Shipping Information Frozen
Preceptrol? no
Genotype Toxinotype: 0
Type Strain 模式菌種 no
Comments 注釋 Preparation of Clostridium difficile strain 630 ATCC? BAA-1382-FZ?, frozen for ease of use. Lot specific titers are available upon request. Toxinotype 0 Ribotype 012 Presence of tcdA and tcdB genes confirmed by PCR. Binary toxin gene cdtB not amplified by PCR Genome sequenced strain
Medium 培養(yǎng)基 ATCC? Medium 2107: Modified Reinforced Clostridial Agar/Broth Medium (pre-reduced)
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度: 37°C
Atmosphere 需氧情況: Anaerobic gas mixture 80% N2 -10% CO2 -10% H2
Genotype Toxinotype: 0
Name of Depositor 寄存人 B Wren, H Maschler
Isolation 分離基物 Clinical isolate Switzerland
References Wust Jurg, et al. Investigation of an Outbreak of Antibiotic-Associated Colitis by Various Typing Methods. J. Clin. Microbiol. 16: 1096-1101, 1982.
Sebaihia Mohammed. The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nat. Genet. 38: 779-786, 2006.
Lemee L, et al. Multiplex PCR targeting tpi (triose phosphate isomerase), tcdA (Toxin A), and tcdB (Toxin B) genes for toxigenic culture of Clostridium difficile. J. Clin. Microbiol. 42(12): 5710-5714, 2004. PubMed: 15583303
Rupnik, M et al. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8): 2240-2247, 1998. PubMed: 9665999
Fawley WN, Wilcox MH; on behalf of the Clostridium difficile Ribotyping Network for England and N. Ireland (CDRN). An enhanced DNA fingerprinting service to investigate potential Clostridium difficile infection case clusters sharing the same PCR-ribotype. J. Clin. Microbiol. 49(12): 4333-4337, 2011. PubMed: 21956986