Zygosaccharomyces rouxii (Boutroux) Yarrow 拉丁名
(ATCC 14679 ) 統(tǒng)一編號
Deposited As Saccharomyces rouxii Boutroux
Strain Designations 別名 NRRL Y-2547 [CBS 4837, CCRC 21487, DSM 2531, IFO 1876, NBRC 1876, NCYC 751]
Alternate State Candida mogii Vidal-Leiria, Candida placentae Goto
Application Produces miso Transformation host
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen 冷凍物: -80°C or colder
Freeze-Dried 凍干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌種 no
Preceptrol? no
Genome Sequenced Strain Yes
Mating Type a
Comments Devoid of any plasmids Miso fermentation Genome sequencing strain (Kikkoman Corporation, Japan)
Medium 培養(yǎng)基 ATCC? Medium 28: Emmons' modification of Sabouraud's agar
ATCC? Medium 200: YM agar or YM broth
ATCC? Medium 336: Potato dextrose agar (PDA)
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTATAGAAAATGACGTGAACTCTTAACGGAGTTC
TCTCAAAGTGTTGGAGGGGAAGGCCTGCGCTTAATTGCGCGGCTGTTTTTAATCTCCTCCGCCTTTGATACAC
ACATTGGAGTTTCTACTTTTTTGTTCTCTTTGGGAGGGTTCTGCTCTCCCAGAGGTAAACACAAACAATCTTTTATTATACTATTA
ACACAGTCAAATGAATTTTAAAAACAAAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAA
CTGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCGGGGGGCATGCCTG
TTTGAGCGTCATTTCCCTCTCAAACTTTACGTTTGGTAGTGAGCGATACTCTACTCTGGAGTTTGCTTGAAAATGGGAGGCCATAGG
CGGAGCTTAGTTTGCGACTGTGCCGAGAGGCATGGGAGCGGCCTAGCCACGAAAAGTCGTATTAGGTTTTACCGACTCGGCGGA
ATAGTGGAGAGGTTTCTTTTTTTTTTATTCTAAGGTAAGCCGTCTGGCTAGACAAAATTCTCAAAGTTTGACCTCAAATCAGGTA
GGATTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAA
ATCTGGTACCTTTCGGTGCCCGAGTTGTAATTTGGAGAGAGTGATTCTGGGACTGGCCCTTGCCTATGTTCCTTGGAACAGGA
CGTCATAGAGGGTGAGAACCCCGTGAGGCGAGGTGATCCAGTTCTTTGTAGAGCGCTCTCGAAGAGTCGAGTTGTTTGGGA
ATGCAGCTCTAAGAGGGTGGTAAATTCCATCTAAAGCTAAATACAGGCGAGAGACCGATAGCGAACAAGTACAGTGA
TGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGGACGTGAAATTGTTGAAAGGGAAGGGCATTTGA
TCAGACATGGTGTTTTGTGCCCCTCGCTCCTCGTGGGTGGGGGAATCTCGCAGCTCACTGGGCCAGCATCAGTTTTGGCGGCA
GGAGAAATCCTCGGGAATGTGGCTCTTGCCTCTGGTAGGAGTGTTATAGCCCGGGGGAATACTGCCAGCTGGGACTGA
GGTATGCGACATTTTGTCAAGGATGTTGGCATAATGGTTATATGCCGC
Name of Depositor 寄存者 NRRL Isolation Japanese miso
Cross References Nucleotide (GenBank) : BBQV00000000 Zygosaccharomyces rouxii strain NBRC 1876, whole genome shotgun sequencing project
References 參考文獻 Smith AK, et al. Preparation of miso. US Patent 2,967,108 dated Jan 3 1961 Hesseltine CW. A millennium of fungi, food, and fermentation. Mycologia 57: 149-197, 1965.
Oshio K, et al. Construction of a host-vector system in the osmophilic haploid yeast Zygosaccharomyces rouxii. J. Ferment. Technol. 66: 481-488, 1988.
Solieri L, et al. Unravelling genomic diversity of Zygosaccharomyces rouxii complex with a link to its life cycle. FEMS Yeast Res.13: 245-258, 2013. PubMed: 23279556
Shepherd A, Piper PW. The Fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts. FEMS Yeast Res.10: 527-534, 2010. PubMed: 20491941
Suezawa Y, Suzuki M, Mori H. Genotyping of a miso and soy sauce fermentation yeast, Zygosaccharomyces rouxii, based on sequence analysis of the partial 26S ribosomal RNA gene and two internal transcribed spacers. Biosci. Biotechnol. Biochem. 72: 2452-2455, 2008. PubMed: 18776675
James SA, et al. Molecular evidence for the existence of natural hybrids in the genus Zygosaccharomyces. FEMS Yeast Res. 5: 747-755, 2005. PubMed: 15851103
