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Escherichia coli (Migula) Castellani and Chalmers 拉丁名

 (ATCC® 39463™) 統(tǒng)一編號(hào)

Classification Enterobacteriaceae, Escherichia 

Strain Designations 別名 KB285 

Application transformation host permitting specialized production processes

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried 

Shipping Information Distributed: freeze-dried 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

Preceptrol® no 

Genotype thi- ppc- 

Type Strain 模式菌種 no 

Comments 注釋 NOTE: The deposited strain does NOT conform to the expected phenotype. It is able to grow on media lacking succinate. 

This host allows control of cell mass while producing a biosynthetic compound via fermentation when transformed with a suitable plasmid. It is best suited for the production of compounds which do not yield a net increase in TCA cycle intermediates, are not products of the glycolytic pathway, and are biosynthetically derived solely from glycolytic intermediates. 

Derived from the strain YMC9 (ATCC 33927) by mud(amp lac)1 insertional mutagenesis and then removal of the beta-lactam resistance. Recommendation for verification: Ability to grow on M9 media + 2 mg/ml glucose + 1 ug/ml vitamin B1 + 0.5 mg/ml succinate. Inability to grow on M9 media + 2 mg/ml glucose + 1 ug/ml vitamin B1. 

Medium 培養(yǎng)基 ATCC® Medium 1065: LB Agar/Broth, Miller 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 37.0°C 

Genotype thi- ppc- Name of Depositor Biotechnica International, Inc., KC Backman, Biotechnica International, Inc.

U.S. Patent Number 5,169,768 

Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. 

Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. 

References Backman KC. Method of biosynthesis of phenylalanine. US Patent 5,169,768 dated Dec 8 1992