Neurospora sitophila Shear et Dodge, teleomorph 拉丁名
(ATCC 36935 ) 統(tǒng)一編號
Strain Designations 別名 NRRL 2884
Application 用途 degrades cellulose produces glucosidase, beta; acid Glucosidase, beta, acid, glucocerebrosidase produces protease nonstructural protein 3 produces single-cell protein SCP produces single-cell protein from cellulose reduces chemical oxygen demand (COD) of potato effluent
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保藏條件
Frozen 冷凍物: -80°C or colder
Freeze-Dried 凍干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌種 no
Preceptrol? no
Mating Type mating type minus
Comments 注釋 Impeller-associated shear effects during fermentation The DNA sequences indicate that this strain is phylogenetically closer to Neurospora intermedia than Neurospora sitophila.
Morphology After 5 days on Neurospora medium at 25°C, colony is low, velutinous, buff-colored. Hyphae hyaline, geniculate. After 2 months, arthroconidia abundant, ellipsoidal or irregularly shaped.
Medium 培養(yǎng)基 ATCC? Medium 336: Potato dextrose agar (PDA)
ATCC? Medium 331: Neurospora culture agar
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACAGAGTTGCAAAACTCCCACAAACCATCGCGAATCT
TACCCGTACGGTTGCCTCGGCGCTGGCGGTCCGGAAAGGCCCTCGGGCCCTCCCGGATCCTCGGGTCTCCCGCTCG
CGGGAGGCTGCCCGCCGGAGTGCCGAAACTAAACTCTTGATATTTTATGTCTCTCTGAGTAAACTTTTAAATAAGTCAAAACTTT
CAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTC
AGTGAATCATCGAATCTTTGAACGCACATTGCGCTCGCCAGTATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCAA
CCATCAAGCTCTGCTT
GCGTTGGGGATCCGCGGCTGTCCGCGGTCCCTCAAAATCAGTGGCGGGCTCGCTAGTCACACCGAGCGTAGTAACT
CTACATCGCTATGGTCGTGCGGCGGGTTCTTGCCGTAAAACCCCCCATTTCTAAGGTTGACCTCGGATCAGGTAGGAATA
CCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCTAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATT
TGAAATCTGGCTTCGGCCCGAGTTGTAATTTGTAGAGGAAGCTTTTGGTGAGGCACCTTCTGAGTCCCCTGGAACGGGGCGCC
ATAGAGGGTG
AGAGCCCCGTATAGTCGGATGCCGATCCAATGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGG
GAGGTAAATTTCTTCTAAAGCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCAC
TTTGAAAAGAGGGTTAAATAGCACGTGAAATTGTTGAAAGGGAAGCGTTTGTGACCAGACTTGCGCCGTTCCGATCA
TCCGGTGTTCTCACCGGTGCACTCGGGACGGCTCAGGCCAGCATCGGTTTTGGCGGGGGGATAAAGGTTCGGGGAACG
TAGCTCCTCCGGGA
GTGTTATAGCCCCGGGCGTAATGCCCTCGCCGGGACCGAGGTTCGCGCATCTGCAAGGATGCTGGCGTAATGGTCATCAACGAC
Morphology After 5 days on Neurospora medium at 25°C, colony is low, velutinous, buff-colored. Hyphae hyaline, geniculate. After 2 months, arthroconidia abundant, ellipsoidal or irregularly shaped.
Name of Depositor 寄存者 NRRL
References 參考文獻(xiàn). . Prog. Biotechnol. 9: 961-964, 1994.
. . Biotechnol. Lett. 14: 863-868, 1992. . . Resour. Conserv. Recycl. 13: 139-146, 1995.
Beuchat LR, Basha SM. Protease production by the ontjom fungus, Neurospora sitophila. Eur. J. Appl. Microbiol. 2: 195-203, 1976.
Karim MI, Sistrunk WA. Efficiency of selected strains of fungi in reducing chemical oxygen demand in wastewater from steam-peeled potatoes. J. Food Process Preserv. 8: 211-218, 1984.
Oguntimein GB, Moo-Young M. Production and properties of beta-glucosidase by Neurospora sitophila. World J. Microbiol. Biotechnol. 7: 4-11, 1991.
