Komagataella pastoris (Guilliermond) Yamada et al. 拉丁名
(ATCC® 28485™) 統(tǒng)一編號
Deposited As Pichia pastoris (Guilliermond) Phaff
Strain Designations 別名 CBS 704 [CCRC 21531, DSMZ 70382, JCM 3650, NCYC 175, NRRL Y-1603]
Application 用途 Degrades ethyl alcohol ethanol Degrades hydrogen peroxide Produces acetaldehyde Produces alcohol oxidase Produces single-cell protein SCP Produces single-cell protein from oxygenated hydrocarbons Produces natural flavor aldehydes Converts ethanol to acetaldehyde
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 生長條件
Frozen 冷凍物: -80°C or colder
Freeze-Dried 凍干物: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain 模式菌種 yes
Preceptrol® no
Genome Sequenced Strain Yes Comments Genome sequencing strain (Institute for Applied Microbiology, University of Natural Ressources and Applied Life Sciences, Vienna, Austria; Massachusetts Institute of Technology, USA) Morphology After 3 days cells are spheroidal to ovoidal, and occur singly or in pairs. Growth is cream-colored, dull to faintly glistening and butyrous.
Medium 培養(yǎng)基 ATCC® Medium 28: Emmons' modification of Sabouraud's agar
ATCC® Medium 200: YM agar or YM broth
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度: 24°C to 26°C
Atmosphere 需氧情況: Typical aerobic
Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GTAGCCAAAACACACTGAGTATAACTTCTAGGCTAATACACATACAACCGATTTATCTAAATCTCCTCCCAACG
GATCTCTTGGCTCTCGCGACGATGAAGAACGCAGCGATCCGCGATACGTCCTGGAAGCCGCCGTGAACCA
TCAATTTTCGAACGCACATTGCATACAGGGAGGGTGGGGTGAGTCAATTCTCACACTCGTGGTCTAGTTTAC
AAAGGATGACTAATAACTTCGTCAGCCCTTACCGTTTTCTCCCCCGCCCCCTCAGA
D1D2 region of the 26S ribosomal RNA gene GCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGTCCACCTTTGAAGTAT
TGACGTGGTTGGGAGAGGTTATTGCACAAGTCGCCTGGAACGGCCGCCAAAGAGGGTGATAGCCCCGTAGCAT
ACAACCAATCTTCCCAGCCGACAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAAGGTGGTAGGGTCCATCAAAG
ACTAAATATCAGCGAGAGACCGATAGCAAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGA
AACAGTGCGTGAAATTGGGGGGAGGGAAGGCAAGGCCACGACAGTGGTCCTGCGC
Morphology After 3 days cells are spheroidal to ovoidal, and occur singly or in pairs. Growth is cream-colored, dull to faintly glistening and butyrous.
Name of Depositor 寄存者 CBS
Chain of Custody 來源國家 ATCC <-- CBS <-- A Guilliermond Isolation Exudate of oak; France
Cross References Nucleotide (GenBank) : JQ070155 D1/D2 region of 28S rRNA gene
Nucleotide (GenBank) : KU729078 ITS including 5.8S rRNA gene
Nucleotide (GenBank) : CABH00000000 Komagataella pastoris DSMZ 70382, whole genome shotgun sequencing project References 參考文獻 Duff SJB. Use of colloidal particles to improve the biocatalytic activity of microorganisms. Agric Biol Chem 54: 1879-1882, 1990.
Duff SJB, Murray WD, Overend RP. Oxygen and temperature effects on acetaldehyde-induced catabolite inactivation in Pichia pastoris. Appl Microbiol Biotechnol 36: 82-86, 1991. Duff SJ, Murray WD. Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde. Biotechnol. Bioeng. 31: 790-795, 1988.
Duff SJ, Murray WD. Oxidation of benzyl alcohol by whole cells of Pichia pastoris and by alcohol oxidase in aqueous and nonaqueous reaction media. Biotechnol. Bioeng. 34: 153-159, 1989.
Duff SJ, Murray WD. Production and application of methylotrophic yeast Pichia pastoris. Biotechnol. Bioeng. 31: 44-49, 1988.
Duff SJB, et al. Production of natural flavor aldehydes from natural source primary alcohols C2-C7. US Patent 4,871,669 dated Oct 3 1989
Duff SJ, Murray WD. Production of flavor aldehydes using nongrowing whole cells of Pichia pastoris. Ann. N.Y. Acad. Sci. 542: 428-433, 1988.
Duff SJB, Murray WD, Overend RP. Factors affecting the yeast-mediated conversion of ethanol to acetaldehyde in batch reactors. Enzyme Microb Technol 11: 770-775, 1989.
Mattanovich D, et al. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. Microb. Cell Fact. 8: 29, 2009.
Murray WD, et al. Catabolite inactivation in the methylotrophic yeast Pichia pastoris. Appl. Environ. Microbiol. 56: 2378-2383, 1990.
Murray WD, et al. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32: 95-100, 1989.
Murray WD, Duff SJB. Metabolism of hydrogen peroxide by Pichia pastoris. Agric. Biol. Chem. 54: 1967-1974, 1990.
Phaff HJ. A proposal for amendment of the diagnosis of the genus Pichia Hansen. Antonie van Leeuwenhoek 22: 113-116, 1956.
Wegner EH. Treatment of make-up water for use in a fermentation process for growth of yeast cells requiring growth factors.
US Patent 4,226,939 dated Oct 7 1980 Yamada Y, et al. The phylogenetic relationships of methanol-assimilating yeasts based on the partial sequences of 18S and 26S ribosomal RNAs: the proposal of Komagataella gen. nov. (Saccharomycetaceae). Biosci. Biotechnol. Biochem. 59: 439-444, 1995. PubMed: 7766181
Kurtzman CP, Robnett CJ. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res. 13: 23-33, 2013. PubMed: 22978764
Kurtzman CP. Komagataella populi sp. nov. and Komagataella ulmi sp. nov., two new methanol assimilating yeasts from exudates of deciduous trees. Antonie van Leeuwenhoek 101: 859-868, 2012. PubMed: 22302468
Kurtzman CP, Robnett CJ. Systematics of methanol assimilating yeasts and neighboring taxa from multigene sequence analysis and the proposal of Peterozyma gen. nov., a new member of the Saccharomycetales. FEMS Yeast Res 10: 353-361, 2010. PubMed: 20522116
Kurtzman CP. Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis. J Ind Microbiol Biotechnol 36: 1435-1438, 2009. PubMed: 19760441
Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov.. FEMS Yeast Res. 8: 939-954, 2008. PubMed: 18671746
