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Blakeslea trispora Thaxter 拉丁名

(ATCC? 14271?) 統(tǒng)一編號(hào)

Strain Designations 別名 NRRL 2456 [CBS 130.59] 

Application 用途 Classroom demonstration Produces beta-carotene 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C 

Live Culture: See Propagation Section 

Type Strain 模式菌種 no 

Preceptrol? no 

Genome Sequenced Strain Yes 

Mating Type plus 

Comments 注釋 Genome sequencing strain (Joint Genome Institute, Department of Energy, USA ) 

Medium 培養(yǎng)基 ATCC? Medium 200: YM agar or YM broth 

ATCC? Medium 325: Malt extract agar (Blakeslee's formula) 

ATCC? Medium 340: Rabbit food agar

ATCC? Medium 336: Potato dextrose agar (PDA) 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 24°C to 26°C

Atmosphere 需氧情況: Typical aerobic 

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGCTCGTCCTGTTACTATCTATTTTACTGTGAACTGTTTTATTTTRCAGCGTTTGAGGGATGTTCTTGTGCT

ATATGGGTAGGCATAAGAAATGTTAACCGAGCTGTAATCAGGCTTAGGCTTGGTACCCTGTTTATATA

CTTTCAATTGATCAGATTTTAAAATGTAACATAGAGTAAAATCTATAAAACAACTTTTAACAACGGATCTCTTG

GCTTTTGCATCGATGAAGAACGTAGCAAATTGCGATAACTAGTGTGAATTGCAAATTCAGTGAATCATCGAGTC

TTTGAACGCATCTTGCGCTCATTGGTATTCCAGTGAGCACGCCTGTTTCAGTATCAAAAACAACCCTCATTCAAA

TTTTTTTTTTGAATGGTCATGAAGGAAGCTAACTTGTTGGCGACCTTTTAAATTGAGTAAGGCCTGAATTTGTTTATCT

AGCCTGAACTTTTTTTTAATATAAAGGAAAGCTCTTGCGACTTGAACTTTGTTGGGGCCTCCCAAATAAAGCTTTT 

D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAAATAACAATGATTTCCCTAGTAACGGCGAGTGAACAGGAAAGAGCTCAAA

GTTGGAACCTGTTTGGTTTAGCCAAACCGGATTGTAAACTGTAGAAGTGTTTTCCTGGCACGCCTAACGAAAAAGT

CCATTGGAACATGGTGACATAGAGGGTGAGAGCCCCGTATTTTGTTAGAGCTGTTGTCTTTTGTGATACACTTTC

AAAGAGTCAGGTTGTTTGGGAATGCAGCCTAAATTGGGTGGTAAATCTCACCTAAAGCTAAATATTGGCAAAAG

ACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGAACTTTGAAAAGAGAGTTAAACAGTATGTGAAA

TTGTTAAAAGGGAACCGTTTGGAGCCAGACTGGCTTGATTGTAATCAATCTAGATYTCGATCTGGATGCACTTG

CAGTCTATGCCTGCCAACGACAATTCATTCTGGAGGAAAAAACTAGGAGGAATGTGGCCCCTCGGGGTGTTATA

GACTTTTAGAAAATACTCTGGTTTGGATTGAGGAACGCAGCAGATGCTATTTGGCGAAGGTGCTGAGTGCTTTTA

GTTTTATTGGTTAGAATTTCTGCTTCGGGTGGTGCTAATCAATACGATTGGAACTCGCTTAGTTCACTTTTTATCTGC

TTAGGTTGTTGGCTTAATGACTCTAAATGA

Name of Depositor 寄存者 NRRL 

Chain of Custody 來源國家 ATCC <-- NRRL <-- G.W. Martin

Isolation 分離基物 Soil, Panama 

Cross References Nucleotide (GenBank) : M36311 B.trispora 5S rRNA. 

References 參考文獻(xiàn) Anderson RF. Method for the production of carotenes. US Patent 2,890,989 dated Jun 16 1959 

Hesseltine CW, Anderson RF. Method of making carotenes and related substances by mixed culture fermentation. US Patent 2,865,814 dated Dec 23 1958 

O'Donnell KL. Zygosporangial types of several Mucoralean families In: Lower fungi in the laboratory. Athens, GA Univ., Georgia. pp. 143-144, 1978. 

Ciegler A, Arnold M, Anderson RF. Microbiological production of carotenoids. IV. Effect of various grains on production of beta-carotene by mated strains of Blakeslea trispora. Appl Microbiol 7: 94-101, 1959. PubMed: 13637891

Kim SW, et al. Enhanced synthesis of trisporic acid and beta-carotene production in Blakeslea trispora by addition of a non-ionic surfactant, Span 20. J. Ferment. Bioeng. 84: 330-332, 1997. 

Anderson RE, et al. Microbiological production of beta-carotene in shaken flasks. Agric. Food Chem. 6: 543-545, 1958. 

Hesseltine CW, Anderson RF. Microbiological production of carotenoids. I. Zygospores and carotene produced by intraspecific and interspecific crosses of Choanephoraceae in liquid media. Mycologia 49: 449-452, 1957. 

Walther G, et al. DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia 30: 11-47, 2013. PubMed: 24027345

Burmester A, et al. Cleavage of beta-carotene as the first step in sexual hormone synthesis in zygomycetes is mediated by a trisporic acid regulated beta-carotene oxygenase. Fungal Genet Biol. 44: 1096-1108, 2007. PubMed: 17822929 

O'Donnell K, et al. Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale. Mycologia 93: 286-296, 2001. 

Voigt K, Wostemeyer J. Phylogeny and origin of 82 Zygomycetes from all 54 genera of the Mucorales and Mortierellales based on combined analysis of actin and translation elongation factor EF-1alpha genes. Gene 270: 113-120, 2001. PubMed: 11404008