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Pochonia chlamydosporia var. chlamydosporia (Goddard) Zare et Gams 拉丁名

(ATCC? MYA-4875?) 統(tǒng)一編號

Strain Designations 別名 123 

Alternate State Metacordyceps chlamydosporia (Evans) Sung et al. 

Application 用途 Biocontrol agent of plant parasitic nematodes. 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

 Product Format 提供形式 frozen 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C 

Live Culture: See Propagation Section 

Type Strain 模式菌種 no 

Preceptrol? no 

Genome Sequenced Strain Yes Comments Egg-parasitic nematophagous fungus. Genome sequencing strain (Donnelly Sequencing Centre, University of Toronto, Canada). 

Morphology On PDA medium at 25°C after 7 days, mycelium white to pale yellow, very dense, raised, velvety. Produces a clear exudates after 7 days. Reverse mustard yellow to bright yellow center with pale yellow margin. Hyphae guttulate. Clamp connections not observed. 

Medium 培養(yǎng)基 ATCC? Medium 307: Cornmeal agar 

ATCC? Medium 336: Potato dextrose agar (PDA)

ATCC? Medium 337: Potato, dextrose, yeast agar (PDY) 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 20°C to 25°C 

Atmosphere 需氧情況: Typical aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. TAACAAGGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACCGAGTTTTCAACTCCCAAACCCCAT

GTGAACTTATACCATTTACAACCGTTGCTTCGGCGGGTTCTCGCCCCGGGCTTTACACCCCGGAACCAGGCG

GCCCGCCGGGGGACCCAAACTCTAGATTTTTATTTTAGCATGTCTGAGTGGAATCATTACAAAATGAATCAAAA

CTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTG

CAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGCCAGTATTCTGGCGGGCATGCCTGTTC

GAGCGTCATTTCAACCCTCAAGCCCCAGCGGTTTGGTGTTGGGGACCGGCGAGTACAGAGGCTTTGGGGAC

TTGTCCCCCTTCCCTCGGCGCCGCCCCCGAAATGAATTGGCGGTCTCGTCGCGGCCTCCTCTGCGTAGTAGC

ACAACCTCGCATCAGGAGCGCGGCGCGGCCACTGCCGTAAAACGCCCAACTTTTTTTAAGAGTTGACCTCGA

ATCAGGTAGGAATACCCGCTGAACTTAAGCATATCAATAAGCGGAGG 

D1D2 region of the 28S ribosomal RNA gene: TTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAG

CTCAAATTTGAAATCTGGCCCCTAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGCGAGGTGCCTTCCGAGT

TCCCTGGAACGGGACGCCATAGAGGGTGAGAGCCCCGTCTGGTTGGATGCCGAGCCTCTGTAAAGCTCCTTCGA

CGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGTCTTCTAAAGCTAAATACCGGCCAGAGACC

GATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAATAGTACGTGAAATTGTT

GAAAGGGAAGCACTTATGACCAGACTTGGACCCGGTGAATCATCCAGCGTTCTCGCTGGTGCACTTTGCCGGGCT

CAGGCCAGCATCAGTTTGCCTCGGGGGATAAAGGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTG

CACAATACCCTGGGGCGGACTGAGGTTCGCGCATCTGCAAGGATGCTGG 

 Morphology On PDA medium at 25°C after 7 days, mycelium white to pale yellow, very dense, raised, velvety. Produces a clear exudates after 7 days. Reverse mustard yellow to bright yellow center with pale yellow margin. Hyphae guttulate. Clamp connections not observed.

Name of Depositor 寄存者 LV Lopez-Llorca Special Collection ATCC 

Chain of Custody 來源國家 TCC <-- LV Lopez-Llorca 

Isolation 分離基物 Eggs from phytopathogenic nematode Heterodera avenae, Sevilla, Spain. 

Cross References Nucleotide (GenBank) : KC171356 ITS including 5.8S rRNA gene 

Nucleotide (GenBank) : KC171327 D1/D2 region of 28S rRNA gene 

References 參考文獻 Lopez-Llorca LV, et al. Expression of serine proteases in egg-parasitic nematophagous fungi during barley root colonization. Fung. Genet. Biol. 47: 342-351, 2010. 

Macia-Vicente JG, Jansson HB, and Lopez-Llorca LV. Assessing fungal root colonization for plant improvement. Plant Signal. Behav. 4(5): 445-447, 2009. 

Palma-Guerrero J, et al. Chitosan increases conidiation in fungal pathogens of invertebrates. Appl. Microbiol. Biotechnol. 87: 2237-2245, 2010.