>美國(guó)ATCC>ATCC菌種類>NTL4
Rhizobium radiobacter (Beijerinck and van Delden) Young et al. 拉丁名
(ATCC® BAA-2240™) 統(tǒng)一編號(hào)
Deposited As Agrobacterium tumefaciens
Strain Designations 菌種別名 NTL4(pZLR4)
Application 用途 Bioreporter for detecting N-acyl homoserine lactone quorum-sensing signals.
Isolation 分離源 This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
Biosafety Level 生物安全等級(jí) 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried
Storage Conditions 保存方法
Frozen 冷凍物 : -80°C or colder
Freeze-Dried 凍干物 : 2°C to 8°C
Live Culture 活菌 : See Propagation Section
Preceptrol® no
Type Strain 模式菌株 no
Comments 注釋 The depositor recommends using media supplemented with 25 mcg/ml gentamicin to select for plasmid retention. Pzlr4 confers resistance to beta lactam antibiotics and gentamicin.
The plasmid also confers acyl-homoserine lactone-inducible production of β-galactosidase (LacZ) activity.
Medium 培養(yǎng)基 ATCC® Medium 1885: LB medium (ATCC medium 1065) with 15 mcg/ml gentamycin
Growth Conditions 生長(zhǎng)條件
Temperature 培養(yǎng)溫度 : 28°C
Atmosphere 需氧情況 : Aerobic
Name of Depositor 寄存人 S Farrand
Isolation 分離源 This strain was constructed in 1996, in which the recombinant plasmid pZLR4 was transformed into strain NTL4.
References 參考文獻(xiàn) Cha C, et al. Production of acyl-homoserine lactone quorum-sensing signals by gram-negative plant-associated bacteria. Mol. Plant Microbe Interact. 11(11): 1119-1129, 1998. PubMed: 9805399