pAL4000 Plasmid in Escherichia coli：拉丁名
(ATCC 77192 ) 統(tǒng)一編號
Depositors DR Helinski
Biosafety Level 生物安全等級 1
Vector Information Size (kb): 13.0 Vector: pAL4000 (plasmid) Promoters: Promoter none Construction: RSF1010, pKW102, pBR322, pUC19 Marker(s):tetR Construct size (kb): 13.0
Features: insert detection: luciferase marker(s): tetR promoter: none replicon: RSF1010 (IncQ) terminator: rpoC Applications promoter-cloning vector vector permitting visual detection of activity in colonies
Comments Restriction digests of the clone give the following sizes (kb): BamHI--12.5; EcoRI--9.0, 2.3, 1.2, 0.6, 0.25; PstI/BglII--10.0, 2.4.
Not self transmissible, but mobilizable by the transfer functions of RK2. Was tested in Pseudomonas aeruginosa PAO1161, Acinetobacter calcoaceticus BD413, Rhizobium meliloti 102F34nal, and Agrobacterium tumefaciens A18.
Promoter-cloning vector permitting visual detection of activity using the promoterless luciferase gene as the reporter. The order of the major features in this plasmid is: rpoC rho-independent transcription terminator - EcoRI/MCS/PstI - translation terminator - luc - BglII - RSF1010 - tetR.
Media 培養(yǎng)基 ATCC Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions 生長條件
Temperature 培養(yǎng)溫度 : 37.0°C
References Greener A, et al. Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of gram-negative bacteria. Genetics 130: 27-36, 1992. PubMed: 1732166
