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pWH1266

(ATCC® 77092™) 

Designations pWH1266 

Depositors W Hillen 

Biosafety Level 1 

Vector Information Size (kb): 8.89 Vector: pWH1266 (plasmid) Construction: pBR322, pWH1277 Marker(s):ampR,tetR Construct size (kb): 8.89 Features: marker(s): ampR, tetR Replicon: pMB1, pWH1277 

Applications shuttle vector 

Comments Restriction digests of the clone give the following sizes (kb): BamHI--8.8; PvuII--4.4 (doublet); AccI--3.8, 2.9, 1.2, 0.9; HincII--4.6, 3.1, 1.1; EcoRI--8.8. 

Contains the following restriction sites (approximate kb from the EcoRI site): BamHI--0.38; PvuII--2.1, 6.6; AccI--0.65, 4.5, 5.7, 6.7; HincII--0.65, 3.7, 8.4. 

The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus. Ampicillin and tetracycline resistance are both expressed in A. calcoaceticus. Shuttle vector. The cryptic plasmid pWH1277 isolated from A. calcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322. 

Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin 

Growth Conditions

Temperature: 37°C 

References Hunger M, et al. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87: 45-51, 1990. PubMed: 2185139 

Shipped frozen

Shipping Information plasmid in E. coli