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平臺編號：bio-84094
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國際編號：ATCC 77092
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拉丁屬名： pWH1266
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規(guī)格：Frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
pWH1266*
pWH1266
(ATCC® 77092™)
Designations pWH1266
Depositors W Hillen
Biosafety Level 1
Vector Information Size (kb): 8.89 Vector: pWH1266 (plasmid) Construction: pBR322, pWH1277 Marker(s):ampR,tetR Construct size (kb): 8.89 Features: marker(s): ampR, tetR Replicon: pMB1, pWH1277
Applications shuttle vector
Comments Restriction digests of the clone give the following sizes (kb): BamHI--8.8; PvuII--4.4 (doublet); AccI--3.8, 2.9, 1.2, 0.9; HincII--4.6, 3.1, 1.1; EcoRI--8.8.
Contains the following restriction sites (approximate kb from the EcoRI site): BamHI--0.38; PvuII--2.1, 6.6; AccI--0.65, 4.5, 5.7, 6.7; HincII--0.65, 3.7, 8.4.
The 2.2 AccI/HincII fragment is essential for replication in A. calcoaceticus. Ampicillin and tetracycline resistance are both expressed in A. calcoaceticus. Shuttle vector. The cryptic plasmid pWH1277 isolated from A. calcoaceticus lwoffi was linearized with PvuII and cloned into the PvuII site of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References Hunger M, et al. Analysis and nucleotide sequence of an origin of DNA replication in Acinetobacter calcoaceticus and its use for Escherichia coli shuttle plasmids. Gene 87: 45-51, 1990. PubMed: 2185139
Shipped frozen
Shipping Information plasmid in E. coli
