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CDC B-3325*
    CDC B-3325*
  • 平臺(tái)編號(hào):bio-84169
  • 國(guó)際編號(hào):ATCC 28942
  • 拉丁屬名: Trichophyton tonsurans Malmsten
  • 規(guī)格:frozen
  • 用途:ATCC原裝進(jìn)口
  • 服務(wù)費(fèi)用:
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  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

 Trichophyton tonsurans Malmsten

28942 ™

 

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An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.

Product category

Fungi

Type strain

No

Genome sequenced strain

Yes

Isolation source

Man

Geographical isolation

England

Product format

Frozen

Storage conditions

-80°C or colder

Specific applications

Biomedical Research and Development Material

Preceptrol

No

Characteristics

Morphology

On PDY medium at 25°C after 11 days, mycelium creamy white to pale yellow, velutinous. Reverse bright yellow to orange. Hyphae hyaline, guttulate. Conidia hyaline, ellipsoidal to clavate, smooth, 6-9 X 2.5-4 µm. Chlamydospores subglobose, 15-16.5 X 7.5-10.5 µm.

Handling information

Medium

ATCC Medium 28: Emmons' modification of Sabouraud's agar/broth

ATCC Medium 200: YM agar or YM broth

ATCC Medium 337: Potato, dextrose, yeast agar (PDY)

Temperature

24-26°C

Atmosphere

Aerobic

Handling procedure

Frozen ampoules packed in dry ice should either be thawed immediately or stored in liquid nitrogen.  If liquid nitrogen storage facilities are not available, frozen ampoules may be stored at or below -70°C for approximately one week.  Do not under any circumstance store frozen ampoules at refrigerator freezer temperatures (generally -20°C).  Storage of frozen material at this temperature will result in the death of the culture.

To thaw a frozen ampoule, place in a 25°C to 30°C water bath, until just thawed (approximately 5 minutes).  Immerse the ampoule just sufficient to cover the frozen material.  Do not agitate the ampoule.

Immediately after thawing, wipe down ampoule with 70% ethanol and aseptically transfer at least 50 µL (or 2-3 agar cubes) of the content onto a plate or broth with medium recommended.

Incubate the inoculum/strain at the temperature and conditions recommended. Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 3-6 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

 

 

Handling notes

Control strain for identification.

Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.

 

Quality control specifications

Sequenced data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence

TGAGCAGATGTGCGCCGGCCGTACCGCCCCATTCTTGTCTACCTTACTCGGTTGCCTCGGCGGGCCGCGCTCTCCCAGGAGAGCCGTTCGGCGAGCCTCTCTTTATAGCGGCTCAACGCTGGACCGCGCCCGCCGGAGGACAGACGCAAAAAAAAATTCTTTCAGAAGAGCTGTCAGTCTGAGCGTTAGCAAGCAAAAATCAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGCATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCAGCCCCTCAAGCCCGGCTTGTGTGATGGACGACCGTCCGGCGCCCCCGTCTCTGGGGGTGCGGGACGCGCCCGAAAAGCAGTGGCCAGGCCGCGATTCCGGCTTTCTAGGCGAATGGGCAACAAACCAGCGCCTC

 

 

D1D2 region of the 28S ribosomal RNA gene

CCTCCCCCGGGGGCCCGAGTTGTAATTTGCAGAGGATGTTTCGGGTGCGGCCGCCGTCTAAGTTCCTTGGAACAGGACGTCAGAGAGGGTGAGAATCCCGTCTTGGGCGGGCGGTCCGCGCCCGTGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGCGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCGGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGGTTAAAAGCACCTTGAAAAGGGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGGCCAGACTCGGGGGCGGGGTTCAGCGGGTGCTCGTCGCCCGTGTATTCCTCGTTTCCCGGGCCAGCATCAGTTTCGACGGCCGGTCAAAGGCCCCCGGAATGTGTCGTCTCTCGGGACGTCTTATAGCCGGGGGTGCAATGCGGCCCGTCGGGAC

 

Verification method

Whole-genome Sequencing

History

Deposited as

Trichophyton tonsurans Malmsten

Synonyms

Trichomyces tonsurans (Malmsten) Guéguen, Oidium tonsurans (Malmsten) Zopf, Oospora tonsurans (Malmsten) Saccardo et Traverso, Trichophyton equinum var. equinum, Trichophyton depilans Mégnin, Trichophyton acuminatum Bodin, Trichophyton crateriformis Saccardo, Trichophyton equinum Gedoelst, Trichophyton flavum Bodin, Trichophyton crateriforme Saccardo, Trichophyton fumatum Saccardo, Trichophyton regulare Saccardo, Trichophyton sulphureum Saccardo et Fox, Trichophyton sulfureum Saccardo et Fox, Trichophyton cerebriforme Saccardo, Trichophyton effractum Saccardo, Trichophyton exsiccatum Uriburu, Trichophyton plicatilis Saccardo, Trichophyton pilosum Saccardo, Trichophyton umbilicatum Saccardo, Trichophyton polygorum Uriburu, Trichophyton plicatile Saccardo, Trichophyton ochropyrraceum Muijs, Bodinia spadix Katoâ, Trichophyton crateriformis var. ochropyrraceum (Muijs) Keller, Trichophyton areolatum Negroni, Trichophyton floriforme Beintema, Trichophyton equinum var. autotrophicum Smith et al., Trichophyton tonsurans subvar. perforans Matsumoto et al.

Depositors

MP English

Type of isolate

Human

Patient gender

Male

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