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Metarhizium anisopliae (Metschnikoff) Sorokin var. anisopliae 拉丁名

(ATCC? 90448?) 統(tǒng)一編號

Strain Designations 別名 M.a. 43 [HRI 275.86] 

Application 用途 Biological control of vine weevil, Otiorhynchus sulcatus 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 freeze-dried 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C 

Live Culture: See Propagation Section

Type Strain 模式菌種 no 

Preceptrol? no

Comments 注釋 Compatibility with pesticides 

Morphology On Blakeslee’s malt extract medium at 25°C after 15 days, two mycelial types were observed. White mycelium, cottony to floccose, dense. Yellowish tan mycelium velutinous to powdery with abundant dark brown to black sporodochium. Reverse creamy white to yellowish tan. Hyphae hyaline, guttulate. Conidia honey brown, elongated ovoid, smooth, single and in chains, 6-8 X 1.5-3 μm. 

Medium 培養(yǎng)基 ATCC? Medium 325: Malt extract agar (Blakeslee's formula) 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度: 24°C to 26°C 

Atmosphere 需氧情況: Typical aerobic 

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AGGGATCATTACCGAGTTATCCAACTCCCAACCCCTGTGAATTATACCTTTAATTGTTGCTTCGGCGGGACTTCGCGCCCGCCGGGGACCC

AAACCTTCTGAATTTTTTAATAAGTATCTTCTGAGTGGTTAAAAAAATGAATCAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGAT

GAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGTCAGTATT

CTGGCGGGCATGCCTGTTCGAGCGTCATTACGCCCCTCAAGTCCCCTGTGGACTTGGTGTTGGGGATCGGCGAGGCTGGTTTTCCAGCGC

AGCCGTCCCTCAAATCAATTGGCGGTCTCGCCGTGGCCCTCCTCTGCGCAGTAGTAAAACACTCGCAACAGGAGCCCGGCGCGGTCCAC

TGCCGTAAAACCCCCCAACTTTTTATAGTTGACCTCGAATCAGGTAGGACTACCCGCTGAACTTAA

D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGTCC

CCAGGGCCCGAGTTGTAATTTGCAGAGGATGCTTTTGGTGAGGTGCCTTCCGAGTTCCCTGGAACGGGACGCCATAGAGGGTGAGAG

CCCCGTCTGGTTGGATACCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCTAAATGGGAGGTATATGT

CTTCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGGGTTAAAT

AGTACGTGAAATTGTTGAAAGGGAAGCACTTATGACCAGACTTGGCCCCGGTGAATCATCCAGCGGTTCCCGTTGGTGCACTTTGCCG

GGGTTCAGGCCAGCATCAGTTCGCTCCGGGGGATAAAGGCTTTGGGAATGTGGCTCCCTCGGGAGTGTTATAGCCCATTGCGCAATA

CCCTGTGGCGGGCTGAGGTTCGCGCTTTATGCAAGGATGCTGGCATAATGGTCATCAGTGA 

Morphology On Blakeslee’s malt extract medium at 25°C after 15 days, two mycelial types were observed. White mycelium, cottony to floccose, dense. Yellowish tan mycelium velutinous to powdery with abundant dark brown to black sporodochium. Reverse creamy white to yellowish tan. Hyphae hyaline, guttulate. Conidia honey brown, elongated ovoid, smooth, single and in chains, 6-8 X 1.5-3 μm. 

Name of Depositor 寄存者 G Zimmermann 

Chain of Custody 來源國家 ATCC <-- G Zimmermann <-- E Muller-Kogler 

Isolation 分離基物 Codling moth larvae, Cydia pomonella, Austria

References 參考文獻(xiàn) Moorhouse ER, et al. Influence of fungicides and insecticides on the entomogenous fungus Metarhizium anisopliae, a pathogen of the vine weevil, Otiorhynchus sulcatus. Biocontrol Sci. Technol. 2: 49-58, 1992.