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Scheffersomyces parashehatae Suh et Zhou 拉丁名

(ATCC® MYA-4654™) 統(tǒng)一編號(hào)

Strain Designations 別名 EH046

Application 用途 Ferments xylose Bioethanol production 

Biosafety Level 生物安全等級(jí) 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C 

Live Culture: See Propagation Section 

Type Strain 模式菌種 no 

Preceptrol® no 

Comments 注釋  Insect associated yeast

Morphology On YM agar at 25°C for 3 days, colonies white to cream, butyrous, glistening, smooth, margin entire. Cells ovoidal to ellipsoidal, 4.5-6 μm x 3-4.5 μm, pseudohyphae not observed, ascospores not observed. 

Medium 培養(yǎng)基 ATCC® Medium 28: Emmons' modification of Sabouraud's agar 

ATCC® Medium 200: YM agar or YM broth

ATCC® Medium 1245: YEPD 

Growth Conditions 生長(zhǎng)條件

Temperature 培養(yǎng)溫度: 20°C to 25°C 

Atmosphere 需氧情況: Typical aerobic

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAA

CTGCGCGGCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTTA

GAAATAAGTTGGGCCAGAGGTTAACCAAACTTCAATTTTATTATTGAATTGTTATTTTATTTTATTTGT

CAATTTGTTGATTAAATTCAAAAAATCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATG

AAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGAACG

CACATTGCGCCCTTTGGTATTCCAGAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAGCCCTCGGG

TTTGGTATTGAGTGATACTCTTAGTCAGACTAGGCGTTTGCTTGAAAAGTATCGGCATGAGTAGTACT

AGATAGTGCTTTCAGGATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGAATTCTTGGTAGTGAAT

TTTTAGTATCATGGCTCTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGATTACCC

GCTGAACTTAAGCATATCAATA

28S large subunit ribosomal RNA gene, partial sequence. GTCTTAGTAACGGCGAGTGAAGCGACAAAAGCTCAAATTTGAAATCTGGCGCCTTCGGTGTCC

GAGTTGTAATTTGAAGAAGGTAACTTTGGAGTTGGCTCTTGTCTATGTTCCTTGGAACAGGACGT

CACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCAACTCTATGTAAAGTGCTTTCGAAGAGTC

GAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGA

GACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAG

TACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGG

GTGGGGCCTCTGCAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTG

GCACTACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTT

GACTAGGATGCTGGCATAATGATCCTAAACCGCCCGTCTT 

Morphology On YM agar at 25°C for 3 days, colonies white to cream, butyrous, glistening, smooth, margin entire. Cells ovoidal to ellipsoidal, 4.5-6 μm x 3-4.5 μm, pseudohyphae not observed, ascospores not observed. 

Name of Depositor 寄存者 SO Suh

Special Collection ATCC 

Chain of Custody 來源國(guó)家 ATCC <-- SO Suh 

Isolation 分離基物 Gut of Odontotaenius disjunctus larvae from rotten log, Bull Run Mt., Broad Run, Virginia, USA 

Cross References Nucleotide (GenBank) : JQ023151 RPB 2 gene 

Nucleotide (GenBank) : JQ023139 RPB 1 gene Nucleotide (GenBank) : HQ651973 D1/D2 region of 28S rRNA gene Nucleotide (GenBank) : HQ999949 D1/D2 region of 28S rRNA gene  

Nucleotide (GenBank) : HQ652052 ITS including 5.8S rRNA gene Nucleotide (GenBank) : HQ999965 ITS including 5.8S rRNA gene 

Nucleotide (GenBank) : KC479681 18S rRNA gene Nucleotide (GenBank) : KC507465 EF-1 alpha gene 

Nucleotide (GenBank) : KC479717 XYL1 gene 

References Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624