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pBShUBC9(S) 拉丁名

(ATCC? 99600?) 統(tǒng)一編號    

Designations 統(tǒng)一別名 pBShUBC9(S) 

GenBank Number U66818 

Species Homo sapiens, human 

Depositors C Wang 

Applications 用途 contains sequence ubiquitin-conjugating enzyme E2I 

Vector Construct size (kb): 4.050000190734863 

Insert DNA: cDNA 

Insert lengths(kb): 1.100000023841858 

Tissue: placenta 

Gene product: ubiquitin-conjugating enzyme E2I [UBC9] 

Target Gene: ubiquitin-conjugating enzyme E2I 

Insert Size (kb) 1.100 

Media 培養(yǎng)基 ATCC? Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Comments 注釋 Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.1; PvuII--2.6, 1.0, 0.45; XbaI--3.1, 0.88. 

The insert contains the following restriction sites (approximate kb from the 5' end): NotI--0.06; AvaI--0.07; StuI--0.48; PvuII--0.94; XbaI--0.98. 

ATCC 99599 and ATCC 99600 are the same for the initial 15 bp upstream of the initiation codon, the CDS, and the 3' UTR. 

The clones differ at the 5' end of the 5' UTR. 

ATCC 99599 contains a 791 bp sequence while ATCC 99600 has a different 72 bp sequence. 

References 參考文獻 Wang ZY, et al. Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. J. Biol. Chem. 271: 24811-24816, 1996. PubMed: 8798754