pCW8 plasmid in E. coli 拉丁名
(ATCC® MBA-86™) 統(tǒng)一編號
Designations pCW8 plasmid in E. coli
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors R. Kopito
Vector Construct size (kb): 4.136
Vector: pRBG4
Vector size (kb): 3.806
Insert Ubiquitin, mutant
Insert size (kb): 0.330 (Ubiquitin, 6HIS and c-myc tag)
Insert site: ClaI/EcoRI
Excise insert: EcoRI Putative ID ubiquitin
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments 注釋 This is a cDNA clone of ubiquitin K48R from Saccharomyces cerevisiae with a 6 HIS, c-myc N-terminal tag cloned into the ClaI/EcoRI sites of the pRBG4 vector.
The plasmid was transformed into E. coli DH10B.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References 參考文獻 Ward CL, et al. Degradation of CFTR by the ubiquitin-proteasome pathway. Cell 83: 121-127, 1995.