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Murid herpesvirus 1 拉丁名

(ATCC VR-1399) 統(tǒng)一編號(hào)

Classification Herpesviridae, Muromegalovirus 

Deposited As Mouse salivary gland virus 

Agent Murid herpesvirus 1 

Strain Smith MSGV Positive for Mycoplasma No 

Biosafety Level 生物安全等級(jí) 2 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen 1 mL per vial 

Storage Conditions Vapor phase of liquid nitrogen 

Comments 注釋 Virus primarily cell-associated.Cryopreserve virus in 8% DMSO (ATCC? 4-X?). 

Effect on Host CPE, cell rounding and degeneration 

Recommended Host SC-1 cells (ATCC? CRL-1404?) 

Growth Conditions 生長(zhǎng)條件 

Temperature 培養(yǎng)溫度 : 37°C 

Recommendations For Infection: This product is produced by co-cultivation of virus with fresh host cells. 

Prepare a bulk cell suspension the day of inoculation. 

Seed culture vessels at 8 x 104 - 1 x 105 cells per cm2. 

Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.01) and then dilute virus in virus growth medium to prepare the virus inoculum. 

Add virus inoculum to culture vessels. 

Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. 

Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. 

Incubation: 2-7 days Effect on Host CPE, cell rounding and degeneration

Name of Depositor 寄存人 WP Rowe, National Institute of Allergy and Infectious Diseases 

Special Collection ATCC 

Source Salivary gland tissues from three naturally infected mice. Derived from VR-194?. Treated to remove Mycoplasma hominis at ATCC?. 

References 參考文獻(xiàn) Selgrade MK, et al. Effects of cell source, mouse strain, and immunosuppressive treatment on production of virulent and attenuated murine cytomegalovirus. Infect. Immun. 33: 840-847, 1981. PubMed: 6270000 

Smith MG. Propagation of salivary gland virus of the mouse in tissue cultures. Proc. Soc. Exp. Biol. Med. 86: 435-440, 1954. PubMed: 13194679