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Scheffersomyces parashehatae Suh et Zhou 拉丁名

(ATCC® MYA-4653™) 統(tǒng)一編號

Strain Designations 別名 EH045 [CBS 12535] 

Application 用途 Ferments xylose Bioethanol production 

Biosafety Level 生物安全等級 1 

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. 

Product Format 提供形式 frozen

Storage Conditions 保藏條件

Frozen 冷凍物: -80°C or colder 

Freeze-Dried 凍干物: 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌種 yes 

Preceptrol® no 

Comments 注釋 Insect associated yeast Morphology On YM agar at 25°C for 3 days, colonies white to cream, butyrous, glistening, smooth, margin entire. Cells ovoidal to ellipsoidal, 4.5-6.75 μm x 3-4.5 μm, pseudohyphae not observed, ascospores not observed. 

Medium 培養(yǎng)基 ATCC® Medium 28: Emmons' modification of Sabouraud's agar 

ATCC® Medium 200: YM agar or YM broth

ATCC® Medium 1245: YEPD

Growth Conditions

Temperature: 20°C to 25°C 

Atmosphere: Typical aerobic 

Sequenced Data 18S ribosomal RNA gene, partial sequence; internal   transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GTAACAAGGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCG

GCGAAAAAACCTTACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTTAGAAATAAGTTGGGCC

AGAGGTTAACCAAACTTCAATTTTATTATTGAATTGTTATTTTATTTTATTTGTCAATTTGTTGATTAAATTCAAAAAA

TCTTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATG

AATTGCAGATTTTCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCTTTGGTATTCCAGAGGGCATGCCTGTTT

GAGCGTCATTTCTCTCTCAAGCCCTCGGGTTTGGTATTGAGTGATACTCTTAGTCAGACTAGGCGTTTGCTTGAAAA

GTATCGGCATGAGTAGTACTAGATAGTGCTTTCAGGATATTTCAATGTATTAGGTTTATCCAACTCGTTGAGAATT

CTTGGTAGTGAATTTTTAGTATCATGGCTCTGCCTTACAAAACAACAAACAAGTTTGACCTCAAATCAGGTAGGAT

TACCCGCTGAACTTAAGCATATC

28S large subunit ribosomal RNA gene, partial sequence. TTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGTCTTAGTAACGGCGAGTGAAGCGACAAA

AGCTCAAATTTGAAATCTGGCGCCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTTGGCTCTT

GTCTATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCCAACTCTATGTAAA

GTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATT

GGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGT

ACGTGAAATTGTTGAAAGGGAAGGGTTTGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTC

TGCAGTTTACTGGGCCAGCATCGGTTTGGACGGTAGGATAATGACATTGGAATGTGGCACTACTTCGGTGGTGTG

TTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGCGTCTTTGACTAGGATGCTGGCATAATGATCC

TAAACCGCCCGTCTT 

Morphology On YM agar at 25°C for 3 days, colonies white to cream, butyrous, glistening, smooth, margin entire. Cells ovoidal to ellipsoidal, 4.5-6.75 μm x 3-4.5 μm, pseudohyphae not observed, ascospores not observed. Name of Depositor SO Suh Special Collection ATCC

Chain of Custody 來源國家 ATCC <-- SO Suh

Isolation 分離基物 Gut of Odontotaenius disjunctus larvae from rotten log, Bull Run Mt., Broad Run, Virginia, USA 

Cross References Nucleotide (GenBank) : JQ023150 RPB 2 gene 

Nucleotide (GenBank) : JQ023138 RPB 1 gene 

Nucleotide (GenBank) : HQ651972, HQ999948 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : HQ652051, HQ999964 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : HQ651936 18S rRNA gene Nucleotide (GenBank) : KC507464 EF-1 alpha gene

Nucleotide (GenBank) : KC479716 XYL1 gene 

References Suh SO, et al. Scheffersomyces parashehatae f.a., sp. nov., Scheffersomyces xylosifermentans f.a., sp. nov., Candida broadrunensis sp. nov., and Candida manassasensis sp. nov., four novel yeasts associated with wood- ingesting insects and their ecological and biofuel implications. Int. J. Syst. Evol. Microbiol. 63: 4330-4339, 2013. PubMed: 24014624