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平臺編號：bio-84425
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國際編號：ATCC VR-1828
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拉丁屬名： Avian leukosis virus
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規(guī)格：frozen
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用途：ATCC原裝進口
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
RAV-2
Avian leukosis virus 拉丁名
(ATCC? VR-1828?) 編號
Classification Retroviridae, Alpharetrovirus, Avian leukosis virus (Subgroup B)
Deposited As Rous-associated virus-B
Agent Avian leukosis virus
Strain 菌株別名 RAV-2
Biosafety Level 安全等級 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen 1.0 mL
Storage Conditions 培養(yǎng)條件
-70°C or colder
Images VR-1828 images
Comments 注釋 Derived by adaptation from primary cell culture product ATCC? VR-658? to UMNSAH/DF-1 cells (ATCC? CRL-12203?). ATCC? VR-658? and ATCC? VR-1828? have not been compared for sequence or infectivity in primary cell culture and continuous tissue culture.
Effect on Host CPE, cell enlargement and vacuolization. May also cause syncytia or cell rounding and sloughing. Recommended Host UMNSAH/DF-1 cells (ATCC? CRL-12203?)
Growth Conditions 培養(yǎng)條件
Temperature 培養(yǎng)溫度 : 37°C
Recommendations for Infection: For best results, infection
should be performed on a 60-80% confluent, 18-48 hour old
cellular monolayer. Prepare dilution of virus in minimum amount of
volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or
serum free medium prior to inoculation. Adsorb virus dilution for 1-
2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking
every 20-30 minutes to redistribute inoculum. End adsorption by
adding virus growth medium.
Incubation: 7-14 days at 37°C in a humidified 5% CO2
atmosphere, until CPE is progressed through 80% of the
monolayer. Media changes may be necessary.
Effect on Host CPE, cell enlargement and vacuolization. May also cause syncytia or cell rounding and sloughing.
Name of Depositor 寄存人 W Okazaki, HG Purchase, Holdenried, E Berstein
References 參考文獻 Morgan HR, Traub W. Natl. Cancer Inst. Monogr. 17: 392, 1964.
Vogt PK. Envelope classification of avian RNA tumor viruses. Bibl. Haematol. 36: 153-167, 1970. PubMed: 4376359
Vogt PK. A heterogeneity of Rous Sarcoma Virus revealed by selectively resistant chick embryo cells. Virology 25: 237-247, 1965. PubMed: 14297211
Himly M, et al. The DF-1 chicken fibroblast cell line: transformation induced by diverse oncogenes and cell death resulting from infection by avian leukosis viruses. Virology 248: 295-304, 1998. PubMed: 9721238
Schaefer-Klein J, et al. The EV-O-derived cell line DF-1 supports the efficient replication of avian leukosis-sarcoma viruses and vectors. Virology 248: 305-311, 1998. PubMed: 9721239
Gao Q et al. Development and application of a multiplex PCR method for rapid differential detection of subgroup A, B, and J avian leukosis viruses. J. Clin. Microbiol. 52(1): 37-44, 2014. PubMed: 24131697
