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pBAD18-Cm 拉丁名

(ATCC? 87396?) 編號

Designations pBAD18-Cm 

Depositors L Guzman, J Beckwith 

Biosafety Level 安全等級 1 

Vector Information Size (kb): 6.0260000228881840 

Vector: pBAD18-Cm (phagemid) 

Promoters: Promoter araC 

Construction: pBAD18, cmlR (pACYC184) 

Marker(s):cmlR,araC 

Construct size (kb): 6.026000022888184 

Features: marker(s): cmlR 

operator: I2 + I1 

operator: O1 

operator: O2 

other: CAP site 

promoter: araC 

promoter for expression: arabinose BAD 

regulator: araC 

replicon: M13 

replicon: pMB1 

MCS: NheI...HindIII 

transcription terminator: rrnB T1 + T2 

Applications 用途 expression vector produces protein arabinose regulator vector containing primer sites useful for sequencing 

Comments 注釋 Restriction digests of the clone give the following sizes (kb): 

EcoRI--3.8, 2.3; HindIII--6.0; PstI--5.0, 1.1. 

Cultures should be grown in minimal media for more reproducible induction of expression. 

Expression is induced in glycerol-containing media by addition of arabinose. 

Expression is repressed by addition of glucose or other catabolites. One of several tightly controlled expression vectors (ATCC 87393-87402) regulated by the arabinose operon. 

The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. 

The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp upstream of the NheI site) 5'-CTGTTTCTCCATACCCGTT-3'; 

and one of two 3' primers: 3' primer 1 (2 - 19 bp downstream of the HindIII site) 5'-CTCATCCGCCAAAACAG-3'; 3' primer 2 (17 - 33 bp downstream of the HindIII site) 5'-GGCTGAAAATCTTCTCT-3'. 

Cloned inserts must provide a translation initiation sequence (ATG) and ribosome binding site for expression. 

Plasmid contains bla (ampR) sequences surrounding the cmlR gene which could promoter recombination if this plasmid is used in combination with other compatible ampR plasmids. 

Recombination can be avoided by the use of recA host strains, or it can be used to advantage to intentionally exchange markers among plasmids. 

Media 培養(yǎng)基 ATCC? Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol 

Growth Conditions 生長條件

Temperature 培養(yǎng)溫度 : 37.0°C 

References 參考文獻 Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087 

Shipped 提供形式 frozen 

Shipping Information Freeze dried Escherichia coli containing the plasmid.