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 A20 [A-20] (ATCC TIB-208) 

Organism Mus musculus, mouse
Cell Type B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease reticulum cell sarcoma
Strain BALB/cAnN
Applications
This cell line is a suitable transfection host.
Storage Conditions liquid nitrogen vapor phase
Karyotype diploid; modal number = 37; range = 33 to 38 Ref
Derivation
The A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse.
Antigen Expression
Antigen expression: I-A+ Ref
Receptor Expression
Fc
Genes Expressed
immunoglobulin (surface, sIg+),I-A+
Cellular Products
immunoglobulin (surface, sIg+)
Tumorigenic Yes
Effects
Yes
Comments
The cells express little surface immunoglobulin when grown in Click's medium; however, they express large amounts when grown in RPMI 1640 medium.
The cells can present both alloantigens and protein antigens.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
0.05 mM 2-mercaptoethanol
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Isotype IgG
Population Doubling Time 18 hrs
Name of Depositor KJ Kim
Deposited As Mus musculus
References
Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843
Glimcher LH, et al. Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells. J. Exp. Med. 155: 445-459, 1982. PubMed: 6460073
Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Mallick-Wood CA, et al. Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase. Proc. Natl. Acad. Sci. USA 93: 9704-9709, 1996. PubMed: 8790395
Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998
Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022
Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843
Cross References
Nucleotide (GenBank) : M58558 Murine sm D small nuclear ribonucleoprotein sequence.
Nucleotide (GenBank) : NM_009226 Mus musculus small nuclear ribonucleoprotein D1 (Snrpd1), mRNA.