FTC-238人類濾泡狀甲狀腺癌細(xì)胞,肺轉(zhuǎn)移
產(chǎn)品類別:人源細(xì)胞系
生長特性:Adherent
細(xì)胞形態(tài):Polymorphic
凍存條件:90%FBS+10%DMSO
FTC-238人類濾泡狀甲狀腺癌細(xì)胞,肺轉(zhuǎn)移簡介
Synonyms: FTC-238
Background: FTC-238 was established from a lung metastasis of a follicular thyroid carcinoma from a 42-year-old male. The cells are polymorphic showing flat polygonal to spindle-like morphologies. Complex chromosomal changes were detected as well as a p53 mutation.
FTC-133 (ECACC Catalogue number 94060901), FTC-236 (ECACC Catalogue number 06030202) and FTC-238 (ECACC Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.
Species: human (Homo sapiens)
Tissue: Thyroid (lung metastasis)
Disease: Human follicular thyroid carcinoma, lung metastasis
Gender: 42-year-old male
Morphology: Polymorphic
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X
CSF1PO: 10,11
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 9,10
THO1: 9.3
TPOX: 9
vWA: 15,18
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Culture Medium: DMEM:Ham's F12(1:1)+ 5%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.