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細(xì)胞CELL>
biobw自建細(xì)胞系>
人絨毛膜滋養(yǎng)層細(xì)胞名稱 HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞)
別稱 HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn
背景描述 The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
種屬 人
年齡(性別) 女;6-12周胚胎
組織來源 胎盤
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
細(xì)胞類型 轉(zhuǎn)化細(xì)胞系
生物安全等級(jí) 2
生長(zhǎng)培養(yǎng)基 RPMI-1640+5% FBS+1% P/S
推薦傳代比例 1:3-1:5
推薦換液頻率 2~3次/周
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件 氣相:空氣,95%;CO2,5%溫度:37℃
致瘤性 No (tested in soft agar and nude mice).
抗原表達(dá)情況 cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha
保藏機(jī)構(gòu) ATCC; CRL-3271