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NCI-BL128 [BL128] 拉丁名
(ATCC? CRL-5947?) 統(tǒng)一編號(hào)
Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast; Epstein-Barr virus (EBV) transformed
Product Format 提供形式 frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 生物安全等級(jí) 2 [Cells contain Herpesvirus]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease normal
Age 60 years
Gender male
Ethnicity Black
Storage Conditions 保藏條件liquid nitrogen vapor phase
Comments 注釋 A cell line derived from pleural effusion cells of a small cell lung cancer from this patient is available as ATCC HTB-120.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%. 
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 106 viable cells/mL. Maintain cell density between 1 x 106 and 3 x 106 viable cells/mL.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density)
Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature 培養(yǎng)溫度 : 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 13
D5S818: 9,12
D7S820: 8,10
TH01: 6,9
TPOX: 8
vWA: 17
Name of Depositor 寄存者 AF Gazdar, JD Minna
Deposited As Homo sapiens
References 參考文獻(xiàn) NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.