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LL 29 (AnHa) 拉丁名 
(ATCC? CCL-134?) 統(tǒng)一編號
Organism Homo sapiens, human
Tissue lung
Cell Type fibroblast
Product Format 提供形式  frozen
Morphology fibroblast
Culture Properties adherent
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease Idiopathic pulmonary fibrosis
Age 26 years
Gender female
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
26 years
Caucasian
female
Complete Growth Medium Ham's F12K medium, 85%; fetal bovine serum, 15% 
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium. 
Briefly rinse the cell layer with 0.25% (w/v) Trypsin-053mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.  Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. 
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature 培養(yǎng)溫度 : 37°C
Name of Depositor 寄存者 RG Crystal
Deposited As Homo sapiens