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23 ScCr [10ScNCr/23]拉丁名 
(ATCC? CRL-2751?) 統(tǒng)一編號
Organism Mus musculus, mouse
Tissue bone marrow
Cell Type macrophage
Product Format 提供形式 frozen
Morphology macrophage
Culture Properties mixed, adherent and suspension
Biosafety Level 生物安全等級 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease FIV
Age 6 months adult
Strain C57BL/10ScN (formerly C57BL/10ScNCr, C57BL/10ScCr)
Storage Conditions liquid nitrogen vapor phase
Derivation
23 ScCr is a bone marrow derived macrophage cell line developed from a mouse strain with a deletion of the TLR4 locus.
Antigen Expression
CD11a, mouse; Mus musculus
CD11b, mouse; Mus musculus
F4/80, mouse
Genes Expressed
Macrophage inflammatory protein-2
tumor necrosis factor alpha (TNF alpha)
Cellular Products
Macrophage inflammatory protein-2
tumor necrosis factor alpha (TNF alpha)
Comments 注釋
The cell line is deficient in toll-like receptor 4 (TLR4). Stimulation with lipoteichoic acid (LTA) induces a five to six fold increase in tumor necrosis factor alpha (TNF alpha) and macrophage inflammatory protein-2 (MIP-2) secretion. No increases in either cytokine were found when cells were stimulated with lipopolysaccharides (LPS).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20% 
Subculturing
Remove 75% of the media. Scrape off the attached cells. Resuspend cells at 2 x 105 viable cells/mL. Transfer into new flasks.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Name of Depositor E Lorenz
Deposited As Mus musculus
References 參考文獻
Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.