產(chǎn)品信息
名稱 HARA-B (人肺鱗狀細胞癌細胞) (STR鑒定正確)
別稱 HARAB
背景描述 該細胞系來源于HARA細胞系,高表達PTHrP。
種屬 人
年齡(性別) 男,57歲
生長特性 貼壁細胞
細胞形態(tài) 上皮細胞樣
細胞類型 腫瘤細胞
腫瘤類型 肺癌細胞
生物安全等級 1
生長培養(yǎng)基 RPMI-1640+10% FBS+1% P/S
推薦傳代比例 1:3-1:4
推薦換液頻率 3-4天/次
倍增時間 ~20-24h
凍存條件
凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO
溫度:液氮
培養(yǎng)條件
氣相:空氣,95%;CO2,5%
溫度:37℃
保藏機構(gòu) KCB; KCB 2016077YJ
Profile:HARA cell (human lung squamouse cell carcinoma) established from bone metastasis in nude mouse.
Background:Delivertive of the HARA cell. This cell line expresses PTHrP highly and the bone metastasis is induced extensively by the injection to the heart chamber of nude mouse. Hypercalcemia is induced in mice by hypodermic transplantation of the cell.
Species:human ,sapiens,Homo
Tissue:squamous cell carcinoma
Genetics:originally human cell but the cell line estableshed from metastatic organ (bone) of mouse
Disease:lung carcinoma
Gender:57,male,Japanese
Morphology:N/A
Growth Mode:adherent
Doubling Time:approx. 24 hrs.
DNA Profile: D5S818:12
D13S317:9
D7S820:12
D16S539:10
VWA:16,17
TH01:7
AM:X,Y
TPOX:8,9
CSF1PO:13
Cell Line Authentication Service
Culture Medium:DMEM+10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Methods for Passages:medium change every 3 days.
Virus Tested :GAPDH(+),CMV(-),EBV(-),HHV6(-),HHV7(-),BKV(-),JCV(-),ADV(-),parvoB19(-),HBV(-),HTLV1(-),HTLV2(-),HIV1(-),HIV2(-),HPV18(-)
Isozyme Analysis:G6PD(typeB), NP, LDH examined. Human not HeLa.
CO2 Conc. 5%