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 Human genomic DNA (HCC1395)     CRL-2324D?    
Product category:Human cells
Product type:Nucleic acid
Derived from:HCC1395 (ATCC CRL-2324)
Organism:Homo sapiens
Type strain :No
Applications:Cancer research
Mass:10 μg
Product format:Frozen
Storage conditions:-20°C or colder
Handling notes
This cell line was initiated on December, 14, 1994 from a patient with a family history of cancer (patient's mother had breast cancer). The cell line took 14 months to establish. The tumor was classified as TNM stage I, grade 3, invasive ductal carcinoma with 0 out of 34 lymph node metastasis. The cells are poorly differentiated and vacuolated. The cells are negative for expression of Her2-neu but positive for expression of p53. HCC1395 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. The cells are positive for expression of estrogen receptors (ER +) and negative for expression of progesterone receptors (PR -). The DNA from an EBV-transformed lymphoblastoid cell line (HCC1395BL) from the same patient is available as ATCC CRL-2325D.

The DNA is available with the following restrictions: 

The CRL-2324 cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor DNA products derived from it may be sold or used for commercial purposes. Nor can the cells nor the DNA be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells and DNA are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 
Any proposed commercial use of the cells, DNA or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771 


Formulation: 1X TE buffer (10 mM Tris-HCL, 1 mM EDTA, pH 8.0)
Concentration:See lot-specific information
Lot Number:See lot-specific information
Cell line description:See lot-specific information
Quality control specifications
Electrophoresis - RNA content
No visible RNA detected in the agarose gel
Electrophoresis - digestion
Tested and verified for PCR amplification and restriction digestion.
History
Depositors
ATCC