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HCT-15 Purified Genomic DNA, Quantitative (KRAS Mutant), 3 μg   CCL-225D
Product category:Human cells
Product type:Nucleic acid
Derived from:HCT-15 (ATCC CCL-225)
Organism:Homo sapiens
Concentration:100 ng/μL
Mass:3 μg
Product format:Frozen
Shipping information:Stored in 1X TE buffer
Storage conditions:-20°C or colder
Comments
DNA taken from HCT-15 (ATCC? CCL-225?) Cell Line and quantitated

Cell Line Description:

The HCT-15 cell line is a colon adenocarcinoma cell line

Spin down before use

Quantified for oncology biomarker KRAS p.G13D via NGS and ddPCR
Quality control specifications
Electrophoresis - molecular weight
≥48 kb (or higher than uppermost band of the high MW DNA ladder)
Electrophoresis - RNA content
No visible RNA detected in the agarose gel
Total amount
Total DNA by PicoGreen? measurement was found to be ≥ 3 μg
STR profiling
Identical STR profile to cell line source
Mutation alleic frequency
Report results: NGS (Coverage > 10,000X)*
Absolute/relative gene copies/ng DNA
Report results: ddPCR? (Average of nine data points)*
Electrophoresis - digestion
Verified by restriction enzyme digestion.
Verification method
ATCC Cell Line Authentication Service
Sanger Sequencing