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 HTR-8/SVneo
CRL-3271?

Product category  Human cells
Organism  Homo sapiens
Cell type  trophoblast
Morphology   epithelial-like
Tissue     Placenta
Applications    3D cell culture
Product format      Frozen
Storage conditions    Vapor phase of liquid nitrogen
General
Specific applications
These transfected trophoblasts may be used for the study of trophoblast biology and placental function. They may improve our understanding of diseases related to tumor progression, abnormal placentation hypoinvasiveness in preeclampsia, and hyperinvasiveness in trophoblastic neoplasms.
Characteristics
Volume
1.0 mL
Growth properties
Adherent
Derivation
The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
Age
6 to 12 weeks gestation
Immortalization method
SV40 large T antigen transformed
Tumorigenic
Yes; No (tested in soft agar and nude mice)
Antigen expression
Cytokeratin-7+; insulin-like growth factor (IGF)-II+;  NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +;  proliferating cell nuclear antigen (PCNA)+;  human leukocyte antigen framework antigen (W6/32)+;  integrin subunits alpha 1, alpha 3, alpha 5, alpha v and beta 1+
Comments
CRL-3271, HTR-8/SVneo, cells were derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. These cells exhibit a variety of markers characteristic of extravillous invasive trophoblast cells in situ: insulin-like growth factor (IGF)-II, NDOG-5, proliferating cell nuclear antigen (PCNA), human leukocyte antigen framework antigen (W6/32) and a distinct set of integrins including alpha 1, alpha 3, alpha 5, alpha v and beta 1 subunits and alpha v beta 3/beta 5 vitronectin receptor. They were negative formacrophage marker 63/D3, endothelial cell marker factor VIII and alpha 6 and beta 4 integrin subunits.  HTR-8/SVneo cells are useful to study trophoblast and placental biology.

Note: This cell line has been shown to contain both epithelial and mesenchymal-like cell populations.