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平臺編號：bio-116148
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國際編號：CRL-3271
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細(xì)胞信息： HTR-8/SVneo
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規(guī)格： Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
HTR-8/SVneo
HTR-8/SVneo
CRL-3271?
Product category Human cells
Organism Homo sapiens
Cell type trophoblast
Morphology epithelial-like
Tissue Placenta
Applications 3D cell culture
Product format Frozen
Storage conditions Vapor phase of liquid nitrogen
General
Specific applications
These transfected trophoblasts may be used for the study of trophoblast biology and placental function. They may improve our understanding of diseases related to tumor progression, abnormal placentation hypoinvasiveness in preeclampsia, and hyperinvasiveness in trophoblastic neoplasms.
Characteristics
Volume
1.0 mL
Growth properties
Adherent
Derivation
The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
Age
6 to 12 weeks gestation
Immortalization method
SV40 large T antigen transformed
Tumorigenic
Yes; No (tested in soft agar and nude mice)
Antigen expression
Cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha 5, alpha v and beta 1+
Comments
CRL-3271, HTR-8/SVneo, cells were derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. These cells exhibit a variety of markers characteristic of extravillous invasive trophoblast cells in situ: insulin-like growth factor (IGF)-II, NDOG-5, proliferating cell nuclear antigen (PCNA), human leukocyte antigen framework antigen (W6/32) and a distinct set of integrins including alpha 1, alpha 3, alpha 5, alpha v and beta 1 subunits and alpha v beta 3/beta 5 vitronectin receptor. They were negative formacrophage marker 63/D3, endothelial cell marker factor VIII and alpha 6 and beta 4 integrin subunits. HTR-8/SVneo cells are useful to study trophoblast and placental biology.
Note: This cell line has been shown to contain both epithelial and mesenchymal-like cell populations.
CRL-3271?
Product category Human cells
Organism Homo sapiens
Cell type trophoblast
Morphology epithelial-like
Tissue Placenta
Applications 3D cell culture
Product format Frozen
Storage conditions Vapor phase of liquid nitrogen
General
Specific applications
These transfected trophoblasts may be used for the study of trophoblast biology and placental function. They may improve our understanding of diseases related to tumor progression, abnormal placentation hypoinvasiveness in preeclampsia, and hyperinvasiveness in trophoblastic neoplasms.
Characteristics
Volume
1.0 mL
Growth properties
Adherent
Derivation
The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen.
Age
6 to 12 weeks gestation
Immortalization method
SV40 large T antigen transformed
Tumorigenic
Yes; No (tested in soft agar and nude mice)
Antigen expression
Cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha 5, alpha v and beta 1+
Comments
CRL-3271, HTR-8/SVneo, cells were derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. These cells exhibit a variety of markers characteristic of extravillous invasive trophoblast cells in situ: insulin-like growth factor (IGF)-II, NDOG-5, proliferating cell nuclear antigen (PCNA), human leukocyte antigen framework antigen (W6/32) and a distinct set of integrins including alpha 1, alpha 3, alpha 5, alpha v and beta 1 subunits and alpha v beta 3/beta 5 vitronectin receptor. They were negative formacrophage marker 63/D3, endothelial cell marker factor VIII and alpha 6 and beta 4 integrin subunits. HTR-8/SVneo cells are useful to study trophoblast and placental biology.
Note: This cell line has been shown to contain both epithelial and mesenchymal-like cell populations.
