產(chǎn)品信息
名稱 DoHH2 (人B細胞淋巴瘤細胞) (STR鑒定正確)
別稱 DOHH2; DoHH-2; DOHH-2
種屬 人源
生長特性 懸浮細胞
細胞形態(tài) 淋巴細胞樣
凍存條件 凍存液：55% 基礎培養(yǎng)基+40% FBS+5% DMSO溫度：液氮
培養(yǎng)方案A（默認） 生長培養(yǎng)基:RPMI-1640＋10% FBS＋1% P/S培養(yǎng)條件:氣相：空氣，95%；CO2，5%, 溫度：37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3次/周
參考資料(來源文獻)
背景描述 established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described in the literature to carry the t(14;18) leading to IGH-BCL2 fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq)
年齡（性別） 男性；60歲
組織來源 胸腔積液
細胞類型 腫瘤細胞
腫瘤類型 淋巴瘤細胞
生物安全等級 BSL-1
倍增時間 ~40 hours
保藏機構 DSMZ; ACC-47
