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平臺編號：bio-129533
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細(xì)胞信息： EFM-192A
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：細(xì)胞系
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
人乳腺癌細(xì)胞EFM-192A
細(xì)胞名稱：人乳腺癌細(xì)胞EFM-192A
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運輸方式：常溫保溫運輸；干冰運輸
安全等級：1
用途限制：僅供科研2類
培養(yǎng)體系：DMEM高糖+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡介：人乳腺癌細(xì)胞EFM-192A取自46歲女性。該細(xì)胞源于DSMZ
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:GrayJW Breast Cancer Cell Line Panel.
Part of:MD Anderson Cell Lines Project.
Doubling time:~9 days(DSMZ);80.21 hours(https://www.synapse.org/#!Synapse:syn2347014).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:CNV analysis.
Omics:Deep exome analysis.
Omics:Deep quantitative proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
基因突變
Heterozygous for PIK3CA p.Cys420Arg(c.1258T>C)(CCLE;Cosmic-CLP).
Heterozygous for TP53 p.Glu271fs*1(c.807_808insT)(CCLE;Cosmic-CLP).
HLA信息
Class I
HLA-A A*01:01,24:02
HLA-B B*07:02,39:01
HLA-C C*12:03,07:02
Class II
HLA-DQ DQB1*06:02,02:02
HLA-DR DRB1*15:01,09:10
STR信息
Amelogenin X
CSF1PO 12,13
D3S1358 16
D5S818 12,13
D7S820 8,11
D8S1179 9,15
D13S317 9,14
D16S539 8,13
D18S51 17
D21S11 32.2
FGA 20,22
Penta D 9
Penta E 14,16
TH01 7
TPOX 8,10
vWA 16
參考文獻(xiàn)
PubMed=28196595;DOI=10.1016/j.ccell.2017.01.005
Li J.,Zhao W.,Akbani R.,Liu W.,Ju Z.,Ling S.,Vellano C.P.,Roebuck P.,Yu Q.,Eterovic A.K.,Byers L.A.,Davies M.A.,Deng W.,Gopal Y.N.V.,Chen G.,von Euw E.M.,Slamon D.J.,Conklin D.,Heymach J.V.,Gazdar A.F.,Minna J.D.,Myers J.N.,Lu Y.,Mills G.B.,Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31978347;DOI=10.1016/j.cell.2019.12.023
Nusinow D.P.,Szpyt J.,Ghandi M.,Rose C.M.,McDonald E.R.III,Kalocsay M.,Jane-Valbuena J.,Gelfand E.,Schweppe D.K.,Jedrychowski M.,Golji J.,Porter D.A.,Rejtar T.,Wang Y.K.,Kryukov G.V.,Stegmeier F.,Erickson B.K.,Garraway L.A.,Sellers W.R.,Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402(2020)
細(xì)胞操作說明書下載：細(xì)胞操作說明書
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運輸方式：常溫保溫運輸；干冰運輸
安全等級：1
用途限制：僅供科研2類
培養(yǎng)體系：DMEM高糖+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡介：人乳腺癌細(xì)胞EFM-192A取自46歲女性。該細(xì)胞源于DSMZ
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:GrayJW Breast Cancer Cell Line Panel.
Part of:MD Anderson Cell Lines Project.
Doubling time:~9 days(DSMZ);80.21 hours(https://www.synapse.org/#!Synapse:syn2347014).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:CNV analysis.
Omics:Deep exome analysis.
Omics:Deep quantitative proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
基因突變
Heterozygous for PIK3CA p.Cys420Arg(c.1258T>C)(CCLE;Cosmic-CLP).
Heterozygous for TP53 p.Glu271fs*1(c.807_808insT)(CCLE;Cosmic-CLP).
HLA信息
Class I
HLA-A A*01:01,24:02
HLA-B B*07:02,39:01
HLA-C C*12:03,07:02
Class II
HLA-DQ DQB1*06:02,02:02
HLA-DR DRB1*15:01,09:10
STR信息
Amelogenin X
CSF1PO 12,13
D3S1358 16
D5S818 12,13
D7S820 8,11
D8S1179 9,15
D13S317 9,14
D16S539 8,13
D18S51 17
D21S11 32.2
FGA 20,22
Penta D 9
Penta E 14,16
TH01 7
TPOX 8,10
vWA 16
參考文獻(xiàn)
PubMed=28196595;DOI=10.1016/j.ccell.2017.01.005
Li J.,Zhao W.,Akbani R.,Liu W.,Ju Z.,Ling S.,Vellano C.P.,Roebuck P.,Yu Q.,Eterovic A.K.,Byers L.A.,Davies M.A.,Deng W.,Gopal Y.N.V.,Chen G.,von Euw E.M.,Slamon D.J.,Conklin D.,Heymach J.V.,Gazdar A.F.,Minna J.D.,Myers J.N.,Lu Y.,Mills G.B.,Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31978347;DOI=10.1016/j.cell.2019.12.023
Nusinow D.P.,Szpyt J.,Ghandi M.,Rose C.M.,McDonald E.R.III,Kalocsay M.,Jane-Valbuena J.,Gelfand E.,Schweppe D.K.,Jedrychowski M.,Golji J.,Porter D.A.,Rejtar T.,Wang Y.K.,Kryukov G.V.,Stegmeier F.,Erickson B.K.,Garraway L.A.,Sellers W.R.,Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402(2020)
細(xì)胞操作說明書下載：細(xì)胞操作說明書
