細(xì)胞名稱:人膀胱癌細(xì)胞VM-CUB-3
產(chǎn)品規(guī)格:T25培養(yǎng)瓶x1;1.5ml凍存管x2
細(xì)胞數(shù)量:1x10^6;1x10^6
保存溫度:37℃;-198℃
運(yùn)輸方式:常溫保溫運(yùn)輸;干冰運(yùn)輸
安全等級(jí):1
用途限制:僅供科研3類
培養(yǎng)體系:DMEM高糖(不含丙酮酸鈉)+10%FBS+1%三抗
培養(yǎng)溫度:37℃
二氧化碳濃度:5%
簡(jiǎn)介:人膀胱癌細(xì)胞VM-CUB-3取自女性供體,貼壁培養(yǎng)。
注釋:Problematic cell line:Partially contaminated.Some stocks are contaminated by VM-CUB-1(PubMed=3708594;PubMed=20143388).Although PubMed=26621286 shows it to be different from VM-CUB-1.
Part of:BLA-40 bladder carcinoma cell line panel.
Part of:UBC-40 urothelial bladder cancer cell line index.
Registration:International Cell Line Authentication Committee,Register of Misidentified Cell Lines;ICLAC-00102.
Doubling time:26 hours(PubMed=3708594).
Omics:CNV analysis.
Omics:Deep exome analysis.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
突變信息
TERT c.1-124C>T(c.228C>T)(C228T);Note=In promoter(PubMed=24035680).
Homozygous for TP53 p.Pro278Leu(c.833C>T)(ClinVar=VCV000232497)(PubMed=27270441).
STR信息
Amelogenin X
CSF1PO 12
D5S818 11
D7S820 8,9
D13S317 9,12
D16S539 12
TH01 9.3
TPOX 8
vWA 16
參考文獻(xiàn)
PubMed=25997541;DOI=10.1186/s12864-015-1450-3
Earl J.,Rico D.,Carrillo-de-Santa-Pau E.,Rodriguez-Santiago B.,Mendez-Pertuz M.,Auer H.,Gomez G.,Grossman H.B.,Pisano D.G.,Schulz W.A.,Perez-Jurado L.A.,Carrato A.,Theodorescu D.,Chanock S.J.,Valencia A.,Real F.X.
The UBC-40 Urothelial Bladder Cancer cell line index:a genomic resource for functional studies.
BMC Genomics 16:403-403(2015)
PubMed=26621286;DOI=10.1186/s12864-015-2227-4
Earl J.,Rico D.,Carrillo-de-Santa-Pau E.,Rodriguez-Santiago B.,Mendez-Pertuz M.,Auer H.,Gomez G.,Grossman H.B.,Pisano D.G.,Schulz W.A.,Perez-Jurado L.A.,Carrato A.,Theodorescu D.,Chanock S.J.,Valencia A.,Real F.X.
Erratum to:The UBC-40 Urothelial Bladder Cancer cell line index:a genomic resource for functional studies.
BMC Genomics 16:1019-1019(2015)
PubMed=27270441;DOI=10.1038/onc.2016.172
Nickerson M.L.,Witte N.,Im K.M.,Turan S.,Owens C.,Misner K.,Tsang S.X.,Cai Z.,Wu S.,Dean M.,Costello J.C.,Theodorescu D.
Molecular analysis of urothelial cancer cell lines for modeling tumor biology and drug response.
Oncogene 36:35-46(2017)
PubMed=29732388;DOI=10.3233/BLC-180167
Zuiverloon T.C.M.,de Jong F.C.,Costello J.C.,Theodorescu D.
Systematic review:characteristics and preclinical uses of bladder cancer cell lines.
Bladder Cancer 4:169-183(2018)
細(xì)胞操作說明書下載:
細(xì)胞操作說明書