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平臺(tái)編號(hào)：bio-129694
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細(xì)胞信息： NCI-H1734
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
人非小細(xì)胞肺癌細(xì)胞NCI-H1734
細(xì)胞名稱：人非小細(xì)胞肺癌細(xì)胞NCI-H1734
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研2類
培養(yǎng)體系：DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人非小細(xì)胞肺癌細(xì)胞NCI-H1734取自56歲女性供體。
注釋
Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:MD Anderson Cell Lines Project.
Part of:TCGA-110-CL cell line panel.
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Deep exome analysis.
Omics:Deep phosphoproteome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
Misspelling:NCI-H734;In Cosmic 897512.
基因突變
Heterozygous for ATM p.Gln65Pro(c.194A>C)(ClinVar=VCV000820386)(PubMed=27602502;CCLE;Cosmic-CLP).
Heterozygous for KRAS p.Gly13Cys(c.37G>T)(ClinVar=VCV000045123)(PubMed=1311061;CCLE;Cosmic-CLP).
Homozygous for RB1 p.Ser127Ile(c.380G>T)(ClinVar=VCV000958692)(CCLE;Cosmic-CLP).
Homozygous for TP53 p.Arg273Leu(c.818G>T)(ClinVar=VCV000376655)(PubMed=1311061;CCLE;Cosmic-CLP).
HLA信息： /
STR信息
Amelogenin X
CSF1PO 13
D3S1358 16
D5S818 10
D7S820 10,11
D8S1179 13
D13S317 11,12
D16S539 12
D18S51 13,15
D21S11 33.2
FGA 24
Penta D 11
Penta E 12,14
TH01 7,9.3
TPOX 11
vWA 17
參考文獻(xiàn)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31395879;DOI=10.1038/s41467-019-11415-2
Yu K.,Chen B.,Aran D.,Charalel J.,Yau C.,Wolf D.M.,van't Veer L.J.,Butte A.J.,Goldstein T.,Sirota M.
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat.Commun.10:3574-3574(2019)
PubMed=31803961;DOI=10.1002/jcb.29564
Mulshine J.L.,Ujhazy P.,Antman M.,Burgess C.M.,Kuzmin I.,Bunn P.A.Jr.,Johnson B.E.,Roth J.A.,Pass H.I.,Ross S.M.,Aldige C.R.,Wistuba I.I.,Minna J.D.
From clinical specimens to human cancer preclinical models--a journey the NCI-cell line database-25 years later.
J.Cell.Biochem.121:3986-3999(2020)
細(xì)胞操作說明下載：細(xì)胞操作說明
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研2類
培養(yǎng)體系：DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人非小細(xì)胞肺癌細(xì)胞NCI-H1734取自56歲女性供體。
注釋
Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:MD Anderson Cell Lines Project.
Part of:TCGA-110-CL cell line panel.
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Deep exome analysis.
Omics:Deep phosphoproteome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
Misspelling:NCI-H734;In Cosmic 897512.
基因突變
Heterozygous for ATM p.Gln65Pro(c.194A>C)(ClinVar=VCV000820386)(PubMed=27602502;CCLE;Cosmic-CLP).
Heterozygous for KRAS p.Gly13Cys(c.37G>T)(ClinVar=VCV000045123)(PubMed=1311061;CCLE;Cosmic-CLP).
Homozygous for RB1 p.Ser127Ile(c.380G>T)(ClinVar=VCV000958692)(CCLE;Cosmic-CLP).
Homozygous for TP53 p.Arg273Leu(c.818G>T)(ClinVar=VCV000376655)(PubMed=1311061;CCLE;Cosmic-CLP).
HLA信息： /
STR信息
Amelogenin X
CSF1PO 13
D3S1358 16
D5S818 10
D7S820 10,11
D8S1179 13
D13S317 11,12
D16S539 12
D18S51 13,15
D21S11 33.2
FGA 24
Penta D 11
Penta E 12,14
TH01 7,9.3
TPOX 11
vWA 17
參考文獻(xiàn)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
PubMed=31395879;DOI=10.1038/s41467-019-11415-2
Yu K.,Chen B.,Aran D.,Charalel J.,Yau C.,Wolf D.M.,van't Veer L.J.,Butte A.J.,Goldstein T.,Sirota M.
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat.Commun.10:3574-3574(2019)
PubMed=31803961;DOI=10.1002/jcb.29564
Mulshine J.L.,Ujhazy P.,Antman M.,Burgess C.M.,Kuzmin I.,Bunn P.A.Jr.,Johnson B.E.,Roth J.A.,Pass H.I.,Ross S.M.,Aldige C.R.,Wistuba I.I.,Minna J.D.
From clinical specimens to human cancer preclinical models--a journey the NCI-cell line database-25 years later.
J.Cell.Biochem.121:3986-3999(2020)
細(xì)胞操作說明下載：細(xì)胞操作說明
