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平臺(tái)編號(hào)：bio-129806
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細(xì)胞信息： A427
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：細(xì)胞系
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
人肺癌細(xì)胞A427
細(xì)胞名稱：人肺癌細(xì)胞A427
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研3類
培養(yǎng)體系：DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗+200ug/ml IL2
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人肺癌細(xì)胞A427取自52歲男性，該細(xì)胞保藏于ATCC
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:MD Anderson Cell Lines Project.
Part of:Naval Biosciences Laboratory(NBL)collection(transferred to ATCC in 1982).
Doubling time:28 hours(in RPMI 1640+10%FBS),38 hours(in ACL-3),42 hours(in ACL-3+BSA)(PubMed=3940644);36.6 hours(PubMed=29681454);~60-70 hours(DSMZ).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Deep exome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
基因突變
Homozygous for CTNNB1 p.Thr41Ala(c.121A>G)(ClinVar=VCV000017580)(ATCC;CCLE).
Heterozygous for KRAS p.Gly12Asp(c.35G>A)(ClinVar=VCV000012582)(PubMed=1855224;PubMed=3945555;ATCC;CCLE;Cosmic-CLP).
HLA信息
/
STR信息
Amelogenin X,Y
CSF1PO 10,12
D3S1358 16
D5S818 12
D7S820 8,12
D8S1179 12,13
D13S317 11,12
D16S539 11,13
D18S51 12
D21S11 31.2
FGA 18
Penta D 13
Penta E 15,17
TH01 9
TPOX 8,11
vWA 17
參考文獻(xiàn)
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
細(xì)胞操作說(shuō)明下載：細(xì)胞操作說(shuō)明
產(chǎn)品規(guī)格：T25培養(yǎng)瓶x1；1.5ml凍存管x2
細(xì)胞數(shù)量：1x10^6；1x10^6
保存溫度：37℃；-198℃
運(yùn)輸方式：常溫保溫運(yùn)輸；干冰運(yùn)輸
安全等級(jí)：1
用途限制：僅供科研3類
培養(yǎng)體系：DMEM高糖（HYCLONE SH30022.01)+10%FBS+1%三抗+200ug/ml IL2
培養(yǎng)溫度：37℃
二氧化碳濃度：5%
簡(jiǎn)介：人肺癌細(xì)胞A427取自52歲男性，該細(xì)胞保藏于ATCC
注釋：Part of:Cancer Cell Line Encyclopedia(CCLE)project.
Part of:COSMIC cell lines project.
Part of:MD Anderson Cell Lines Project.
Part of:Naval Biosciences Laboratory(NBL)collection(transferred to ATCC in 1982).
Doubling time:28 hours(in RPMI 1640+10%FBS),38 hours(in ACL-3),42 hours(in ACL-3+BSA)(PubMed=3940644);36.6 hours(PubMed=29681454);~60-70 hours(DSMZ).
Microsatellite instability:Stable(MSS)(Sanger).
Omics:Deep exome analysis.
Omics:Deep proteome analysis.
Omics:Deep RNAseq analysis.
Omics:DNA methylation analysis.
Omics:Protein expression by reverse-phase protein arrays.
Omics:SNP array analysis.
Omics:Transcriptome analysis.
基因突變
Homozygous for CTNNB1 p.Thr41Ala(c.121A>G)(ClinVar=VCV000017580)(ATCC;CCLE).
Heterozygous for KRAS p.Gly12Asp(c.35G>A)(ClinVar=VCV000012582)(PubMed=1855224;PubMed=3945555;ATCC;CCLE;Cosmic-CLP).
HLA信息
/
STR信息
Amelogenin X,Y
CSF1PO 10,12
D3S1358 16
D5S818 12
D7S820 8,12
D8S1179 12,13
D13S317 11,12
D16S539 11,13
D18S51 12
D21S11 31.2
FGA 18
Penta D 13
Penta E 15,17
TH01 9
TPOX 8,11
vWA 17
參考文獻(xiàn)
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)
PubMed=30894373;DOI=10.1158/0008-5472.CAN-18-2747
Dutil J.,Chen Z.,Monteiro A.N.,Teer J.K.,Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res.79:1263-1273(2019)
PubMed=31068700;DOI=10.1038/s41586-019-1186-3
Ghandi M.,Huang F.W.,Jane-Valbuena J.,Kryukov G.V.,Lo C.C.,McDonald E.R.III,Barretina J.,Gelfand E.T.,Bielski C.M.,Li H.,Hu K.,Andreev-Drakhlin A.Y.,Kim J.,Hess J.M.,Haas B.J.,Aguet F.,Weir B.A.,Rothberg M.V.,Paolella B.R.,Lawrence M.S.,Akbani R.,Lu Y.,Tiv H.L.,Gokhale P.C.,de Weck A.,Mansour A.A.,Oh C.,Shih J.,Hadi K.,Rosen Y.,Bistline J.,Venkatesan K.,Reddy A.,Sonkin D.,Liu M.,Lehar J.,Korn J.M.,Porter D.A.,Jones M.D.,Golji J.,Caponigro G.,Taylor J.E.,Dunning C.M.,Creech A.L.,Warren A.C.,McFarland J.M.,Zamanighomi M.,Kauffmann A.,Stransky N.,Imielinski M.,Maruvka Y.E.,Cherniack A.D.,Tsherniak A.,Vazquez F.,Jaffe J.D.,Lane A.A.,Weinstock D.M.,Johannessen C.M.,Morrissey M.P.,Stegmeier F.,Schlegel R.,Hahn W.C.,Getz G.,Mills G.B.,Boehm J.S.,Golub T.R.,Garraway L.A.,Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
細(xì)胞操作說(shuō)明下載：細(xì)胞操作說(shuō)明
