產(chǎn)品信息
名稱 SVCT (人乳腺上皮細(xì)胞) (STR鑒定正確)
種屬 人類
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度:液氮
培養(yǎng)方案A(默認(rèn)) 生長(zhǎng)培養(yǎng)基:DMEM+2mM Glutamine+5μg/mL Hydrocortisone+10μg/mL Insulin+10% FBS+1% P/S培養(yǎng)條件:氣相:空氣,95%;CO2,5%, 溫度:37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.
年齡(性別) 女性
組織來(lái)源 乳腺
細(xì)胞類型 轉(zhuǎn)化細(xì)胞系
生物安全等級(jí) BSL-1
保藏機(jī)構(gòu) KCB; KCB 2015018YJ