產(chǎn)品信息
名稱(chēng) HCC1954 (人乳腺導(dǎo)管癌細(xì)胞) (STR鑒定正確)
別稱(chēng) HCC-1954; Hamon Cancer Center 1954
種屬 人
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣,有空泡
生長(zhǎng)培養(yǎng)基 RPMI-1640+10% FBS+1% P/S
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度:液氮
培養(yǎng)條件 氣相:空氣,95%;CO2,5%溫度:37℃
推薦傳代比例 1:4-1:8
推薦換液頻率 2-3次/周
參考資料(來(lái)源文獻(xiàn))
背景描述 HCC1954 is an epithelial cell line isolated from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old, Asian female in 1995. HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). Her2/neu is overexpressed in the ELISA assay.
年齡(性別) 女,61歲
組織來(lái)源 Breast; Duct; Mammary gland
細(xì)胞類(lèi)型 腫瘤細(xì)胞
腫瘤類(lèi)型 乳腺癌細(xì)胞
生物安全等級(jí) 1
倍增時(shí)間 45 hours (PubMed=25984343); 43.81 hours (GrayJW panel)
受體表達(dá)情況 Estrogen receptor, not expressed; progesterone receptor, not expressed
基因表達(dá)情況 epithelial glycoprotein 2 (EGP2); cytokeratin 19
保藏機(jī)構(gòu) ATCC; CRL-2338 BCRJ; 0280 KCLB; 9S1954
STR鑒定
STR位點(diǎn)信息
Amelogenin X
CSF1PO 10
D2S1338 17,26
D3S1358 15,16
D5S818 11
D7S820 10,11
D8S1179 12,15
D13S317 8,9
D16S539 9,11
D18S51 14,18
D19S433 13,15.2
D21S11 28,32.2
FGA 22,23
PentaD 9,12
PentaE 12,16
TH01 6,7
TPOX 8,9
vWA 18,19
D6S1043 11,14
D12S391 22
D2S441 10,11