產(chǎn)品信息
名稱 Mino (人外周血套細(xì)胞淋巴瘤細(xì)胞) (STR鑒定正確)
別稱 MINO
種屬 人
生長(zhǎng)特性 懸浮細(xì)胞
細(xì)胞形態(tài) 淋巴母細(xì)胞樣
生長(zhǎng)培養(yǎng)基 RPMI-1640+20% FBS+1% P/S
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件 氣相:空氣,95%;CO2,5%溫度:37℃
推薦傳代比例 1:2 - 1:3
推薦換液頻率 2-3次/周
參考資料(來源文獻(xiàn))
背景描述 Mino cells are lymphoblasts isolated from the peripheral blood of a 64-year-old, White male with mantle cell lymphoma. It can be used for immunology research. The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL. Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1).
年齡(性別) 男性,64歲
組織來源 外周血
細(xì)胞類型 腫瘤細(xì)胞
腫瘤類型 淋巴瘤細(xì)胞
生物安全等級(jí) 1
倍增時(shí)間 40-60 hours
抗原表達(dá)情況 CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC)
基因表達(dá)情況 cyclin D2; cyclin D3; p53; p16 (INK4a); p21(WAF1)
保藏機(jī)構(gòu) AddexBio; C0003033/4947 ATCC; CRL-3000 DSMZ; ACC-687
STR鑒定
STR位點(diǎn)信息
Amelogenin X, Y
CSF1PO 9,11
D2S1338 23
D3S1358 18
D5S818 11,12
D7S820 10,11
D8S1179 11,13
D13S317 12
D16S539 11,12
D18S51 12,14
D19S433 15
D21S11 29,30
FGA 23,25
PentaD 12,13
PentaE 5,17
TH01 9.3
TPOX 8,11
vWA 14,17
D6S1043 11,12
D12S391 18,21
D2S441 12,14