產(chǎn)品信息
名稱(chēng) OE19 (人食管癌細(xì)胞) (STR鑒定正確)
別稱(chēng) OE-19; JROECL 19; JROECL19; OEC19
種屬 人
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
生長(zhǎng)培養(yǎng)基 RPMI-1640+10% FBS+1% P/S
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度:液氮
培養(yǎng)條件 氣相:空氣,95%;CO2,5%溫度:37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3天/次
參考資料(來(lái)源文獻(xiàn))
背景描述 OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2 . All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC.
年齡(性別) 男,72歲
組織來(lái)源 食管癌
細(xì)胞類(lèi)型 腫瘤細(xì)胞
腫瘤類(lèi)型 食管癌細(xì)胞
生物安全等級(jí) 1
倍增時(shí)間 ~50-60 hours (DSMZ=ACC-700)
致瘤性 裸小鼠致癌
保藏機(jī)構(gòu) ECACC; 96071721
STR鑒定
STR位點(diǎn)信息
Amelogenin X
CSF1PO 11,13
D2S1338 18,19
D3S1358 15,18
D5S818 11,14
D7S820 8
D8S1179 13,15
D13S317 9,11
D16S539 12,13
D18S51 12
D19S433 13
D21S11 30
FGA 23,26
PentaD 9
PentaE 5,8
TH01 8,9
TPOX 8
vWA 16,17,18
D6S1043 19
D12S391 20
D2S441 14,15