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平臺編號：bio-132807
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細(xì)胞信息： PNT1A
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：/
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
PNT1A（人正常成人前列腺上皮細(xì)胞（SV40））
PNT1A（人正常成人前列腺上皮細(xì)胞（SV40））
產(chǎn)品類別：人源細(xì)胞系
Synonyms:PNT1A
Background:Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.
Species:human (Homo sapiens)
Tissue:prostate
Disease:Human post pubertal prostate normal, immortalised with SV40
Gender:35 year old male
Morphology:Epithelial
Growth Mode:Adherent
Doubling Time:N/A
DNA Profile: Cell Line Authentication Service
Culture Medium:RPMI 1640 + 10% FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Karyotype:Aneuploid
Subculture Routine:Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C.
產(chǎn)品類別：人源細(xì)胞系
Synonyms:PNT1A
Background:Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.
Species:human (Homo sapiens)
Tissue:prostate
Disease:Human post pubertal prostate normal, immortalised with SV40
Gender:35 year old male
Morphology:Epithelial
Growth Mode:Adherent
Doubling Time:N/A
DNA Profile: Cell Line Authentication Service
Culture Medium:RPMI 1640 + 10% FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Karyotype:Aneuploid
Subculture Routine:Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37°C.
