SV40LT-SMC Clone HEP-SA (大鼠平滑肌細(xì)胞)
產(chǎn)品類別:大鼠細(xì)胞系
凍存條件:90%FBS+10%DMSO
Synonyms:SV40LT-SMC Clone HEP-SA (大鼠平滑肌細(xì)胞)
Background:This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
Species:Rattus norvegicus, rat
Tissue:aorta; smooth muscle
Gender:3 months, rat
Morphology:fibroblast
Growth Mode:adherent
DNA Profile:Cell Line Authentication Service
Culture Medium:DMEM+10%NBCS+200 mcg/mL G418
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Genes Expressed:alpha actin; large T antigen
Cellular Products:alpha actin; large T antigen
Comments:This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen. Transfection was done using conditioned medium from psiTEX cells (which produce a helper free replication defective retrovirus that carries the sequences for large T antigen and neomycin (G418) resistance).
Clone HEP-SA cells maintain smooth muscle cell characteristics including smooth cell alpha actin expression and growth inhibition by heparin. Large T antigen is detectable in the nuclei.