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平臺(tái)編號(hào)：bio-132838
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細(xì)胞信息： NTERA-2 cl.D1
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
NTERA-2 cl.D1（人睪丸癌細(xì)胞）
NTERA-2 cl.D1（人睪丸癌細(xì)胞）
產(chǎn)品類別：人源細(xì)胞系
細(xì)胞形態(tài)：epithelial-like,differentiation changes phenotype
凍存條件：90%FBS+10%DMSO
Synonyms:NTERA-2 cl.D1（人睪丸癌細(xì)胞）
Background:The parental NTERA-2 lines was established in 1980 from a nude mouse xenograft of the Tera-2 cell line (see ATCC HTB-106). The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line.
Species:Homo sapiens, human
Tissue:testis; derived from metastatic site: lung
Disease:malignant pluripotent embryonal carcinoma
Gender:Caucasian，male，22 years
Morphology:epithelial-like,differentiation changes phenotype
Growth Mode:adherent
DNA Profile:Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12,13
D5S818: 9,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 18,19
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Culture Medium:DMEM +10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium:90%FBS+10%DMSO
Comments:This clone differentiates along neuroectodermal lineages after exposure to retinoic acid (RA) or hexamethylene bisacetamide (HMBA).The RA induced differentiation is characterized by glycolipid changes, appearance of neurons, and induction of homeobox (HOX) gene clusters.
The cells exhibit high expression of N-myc oncogene activity.To induce differentiation, the cells should be trypsinized and seeded at a density 1 X 10 exp6 cells per 75 sq. cm. in medium containing 0.01 mM trans-retinoic acid. Stock solutions of trans-retinoic acid (10 mM, dissolved in DMSO) should be stored frozen (preferably under a nitrogen atmosphere).
