L-363(人多發(fā)性骨髓瘤細(xì)胞)
產(chǎn)品類(lèi)別:人源細(xì)胞系
細(xì)胞形態(tài):single, large, round or oval cells in suspension;
凍存條件:90%FBS+10%DMSO
Synonyms:L-363(人多發(fā)性骨髓瘤細(xì)胞)
Background:established from the peripheral blood of a 36-year-old woman with plasma cell leukemia (IgG) in 1977; cells were described in the literature to be EBNA-negative and to express mRNA for proto-oncogene BCL2; plasma cell leukemia is related to multiple myeloma
Species:human (Homo sapiens)
Tissue:N/A
Disease:plasma cell leukemia
Gender:N/A
Morphology:single, large, round or oval cells in suspension;
Growth Mode:N/A
Doubling Time:ca. 25 hours
DNA Profile:N/A
Cell Line Authentication Service
Culture Medium:RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium .
Cryopreservation medium:90%FBS+10%DMSO
Mycoplasma:contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image
Fingerprint:multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:confirmed as human with IEF of AST, LDH, PEP B
Cytogenetics:human hyperdiploid karyotype with 11% polyploidy - 49(45-50)X, -X, -X, -6, +7, +8, +11, +11, +14, +19, -22, t(5;8)(q12/13;q24.32), der(8)(t(5;8)(q12/13;q24.32), der(8)t(7;8)(q11.2;p22), del(11)(q13)x2, add(14)(q32), del(17)(p12), add(19)(q13) - sideline with add(13)(q34) - 11q13 and 14q32 breakpoints recurrent in PCL - resembles published karyotype
Virus ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -