JOSK-I(人組織細(xì)胞淋巴瘤細(xì)胞)
產(chǎn)品類別:人源細(xì)胞系
凍存條件:90%FBS+10%DMSO
Cell Line Name:JOSK-I(人組織細(xì)胞淋巴瘤細(xì)胞)
Cell type:Histiocytic lymphoma
Species:Homo sapiens,human
Description:Supposedly "established from the peripheral blood of a 72-year-old woman with acute myelomonocytic leukemia (AML FAB M4) in 1983"; however DSMZ DNA fingerprinting and cytogenetic analysis established unequivocally that JOSK-I, JOSK-M and U-937 are identical; thus, JOSK-I is clearly a derivative of U-937
Morphology:Single, round to polygonaal cells growing in suspension
Mycoplasma:Negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD68 +, HLA-DR +
Culture Medium:DMEM+10%FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP)
We strongly suggest to purchase the complete medium from .
Cytogenetics:Human hyperdiploid karyotype with 3.5% polyploidy - 56(52-56)Xder(X), +2, +4, +6, +7, +8, +12, -15, +17, +19, +20, +mar, der(X)t(X;3)(q11;p13)t(1;3)(q11;p25), del(1)(p11p22), t(1;5)(p21.1;q23), t(2;18)(p17;q23), del(4)(q22), add(6)(p23), del(7)(q21)/der(7)t(7;18)(q11.23;p11), der(10)t(10;11)(p14;q22.3)/der(10)t(10;11) (p14;q22.3)dup(10)(q25.1), der(11)t(10;11)(p14;q22.3), add(13)(p13), der(17)t(17;18)(p11;q12) - (mar resembles Ph) - carries uncommon 10;11 translocation associated with AML M5
Viruses:ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -