JJN-3(人白血病細(xì)胞)
產(chǎn)品類別:人源細(xì)胞系
生長特性:貼壁生長
細(xì)胞形態(tài):single round to oval cells showing a morphological
凍存條件:90%FBS+10%DMSO
Synonyms:
JJN-3(人白血病細(xì)胞)
Background:
established from the bone marrow of a 57-year-old woman with plasma cell leukemia (IgA1kappa) at diagnosis in 1987; JJN-3 is a subclone of the parental cell line JJN-1; plasma cell leukemia is related to multiple myeloma
Species:
human (Homo sapiens)
Tissue:
N/A
Disease:
plasma cell leukemia
Gender:
N/A
Morphology:
single round to oval cells showing a morphological range from mononuclear to occasionally multinucleated cells growing in suspension;
Growth Mode:
N/A
Doubling Time:
ca. 20-35 hours
DNA Profile:
N/A
Cell Line Authentication Service
Culture Medium:
DMEM:IMDM(1:1)+20%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium:
90%FBS+10%DMSO
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, cykappa +, sm/cylambda -; image
Fingerprint:
multiplex PCR of minisatellite markers revealed a unique DNA profile
Species:
confirmed as human by cytogenetics
Cytogenetics:
human flat-moded hypotriploid karyotype with 9% polyploidy - 58-67XX, +1, -2, +5, +8, +8, -9, -11, -12, -13, -15, -17, +20, add(1)(p22), der(1)t(1;?3)(q41;p26)x2, add(3)(p26), add(5)(p15)x1-2, i(5p), del(6)(q13),del(7)(q32), der(7)t(7;11)(q36;q13), add(8)(p1?)x2, t(12;19)(p13;q13), der(14)add(14)(p11)t(14;16)(q32;q23), der(14)t(14;16)(q32;q23), der(16)t(14;16)(q32;q23)x2, del(22)(q12) - carries two copies of t(14;16) associated with c-MAF activation - resembles published karyotype
Virus
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -