產(chǎn)品信息
名稱 HCC70 (人乳腺導(dǎo)管癌細(xì)胞) (STR鑒定正確)
別稱 HCC-70; HCC 70; HCC0070; Hamon Cancer Center 70
種屬 人
生長(zhǎng)特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
生長(zhǎng)培養(yǎng)基 RPMI-1640+10% FBS+1% P/S
凍存條件 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件 氣相:空氣,95%;CO2,5%溫度:37℃
推薦傳代比例 1:4 -1:6
推薦換液頻率 2-3次/周
參考資料(來源文獻(xiàn))
背景描述 HCC70 is an epithelial cell line isolated from a primary ductal carcinoma from a 49-year-old, Black female in 1992, and the cells took 44 months to establish. HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19.HCC70 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 (EGP2) and for cytokeratin 19. However, recent studies performed outside of ATCC have given conflicting results about the status of the estrogen receptor for these cells. The cells are poorly differentiated. The cells overexpress p53, and are negative for the expression of Her2-neu oncogenes. The depositor of CRL-2315 initially reported the cells were positive for estrogen receptors.
年齡(性別) 女性,49歲
組織來源 乳腺
細(xì)胞類型 腫瘤細(xì)胞
腫瘤類型 乳腺導(dǎo)管癌
生物安全等級(jí) 1
倍增時(shí)間 93 hours (PubMed=25984343)
基因表達(dá)情況 epithelial glycoprotein 2 (EGP2); cytokeratin 19
保藏機(jī)構(gòu) ATCC; CRL-2315