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平臺編號：bio-132982
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拉丁屬名： DMS 114
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
人小細(xì)胞肺癌細(xì)胞
產(chǎn)品信息
名稱 DMS 114 (人肺癌細(xì)胞) (STR鑒定正確)
別稱 DMS-114; DMS114; Darmouth Medical School 114
種屬 人
生長特性 貼壁細(xì)胞
細(xì)胞形態(tài) 上皮細(xì)胞樣
生長培養(yǎng)基 Waymouth's MB 752/1+10%FBS＋1% P/S
凍存條件 凍存液：55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO溫度：液氮
培養(yǎng)條件 氣相：空氣，95%；CO2，5%溫度：37℃
推薦傳代比例 1:2-1:4
推薦換液頻率 2-3次/周
參考資料(來源文獻(xiàn))
背景描述 The line was established from cells from a mediastinal biopsy of a patient with small cell carcinoma of the lung. The patient had not received prior therapy.The patient had not received prior therapy. The cells express HLA class I and class II antigens. Early passages of the cells were contaminated with a bovine mycoplasma (Acholeplasma laidlawii) which was cured (prior to cryopreservation) with A. laidlawii antiserum and kanamycin.
年齡（性別） 男，68歲
組織來源 肺
細(xì)胞類型 腫瘤細(xì)胞
腫瘤類型 肺癌細(xì)胞
生物安全等級 1
倍增時間 3.8 days (PubMed=6266631)
致瘤性 Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
抗原表達(dá)情況 Leu 7 +; My23 +; CD11b +
基因表達(dá)情況 adrenocorticotropin (adrenocorticotropic hormone, ACTH); bombesin; glucagon; 17 beta estradiol; oxytocin - neurophysin (OT-NP)
保藏機構(gòu) ATCC; CRL-2066
STR鑒定
STR位點信息
Amelogenin X
CSF1PO 10,11
D2S1338 17
D3S1358 14,17
D5S818 12
D7S820 10,11
D8S1179 12,13
D13S317 13
D16S539 12
D18S51 18
D19S433 13
D21S11 29
FGA 21
PentaD 11,13
PentaE 7,13
TH01 8,9.3
TPOX 8
vWA 16,17
D6S1043 12,17
D12S391 18
D2S441 10
