HCC44人非小細(xì)胞肺癌細(xì)胞
產(chǎn)品類別:人源細(xì)胞系
生長特性:N/A
細(xì)胞形態(tài):adherent epitheloid cells growing as monolayer;
凍存條件:90%FBS+10%DMSO
Synonyms: HCC44人非小細(xì)胞肺癌細(xì)胞
Background: established from the lung of a 54-year-old woman with non-small cell lung cancer of the adenocarcinoma type
Species: human (Homo sapiens)
Tissue: N/A
Disease: non-small cell lung carcinoma
Gender: N/A
Morphology: adherent epitheloid cells growing as monolayer;
Growth Mode: N/A
Doubling Time: ca. 30 hours
DNA Profile: N/A
Cell Line Authentication Service
Culture Medium: RPMI-1640+10%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: contamination was eliminated with sparfloxacin, then negative in microbiological culture and PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human by cytogenetics
Cytogenetics: human flat-moded hyperpentaploid karyotype with 8% polyploidy - 120-124<5n>XX, +1, +1, +3, +3, -4, -4, -5, +7, +7, +7, -9, -9, -10, +11, -18, -21, -21, +22, +22, +22, +7-14mar, der(1)t(X;1)(q11;p11)x2, del(1)(q21)x2, i(1q), t(1;3)(p11;q11), add(2)(q?11), del(2)(p11), add(3)(q28), add(3)(p11), i(5p), add(8)(p2?), add(8)(q2?), add(8)(q24), der(9;19)(q10;q10)x2, add(10)(q2?5), add(11)(p13), der(11)add(11)(p1?1)add(11)(q25), add(12)(p13), add(16)(p11), add(17)(p1?2), add(18)(p11), del(20)(q12)x1-2, add(20)(q13)x2 - 2-6 dmin present in 80% cells
Virus PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -