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平臺(tái)編號(hào)：bio-133055
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細(xì)胞信息： GPH1
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：/
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GPH1豚鼠心肌細(xì)胞
GPH1豚鼠心肌細(xì)胞
產(chǎn)品類別：其它動(dòng)物細(xì)胞系
細(xì)胞形態(tài)：貼壁上皮細(xì)胞融合生長
凍存條件：90%FBS+10%DMSO
Synonyms: GPH1豚鼠心肌細(xì)胞
Background: originally described as a glioma cell line established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG; described in the literature to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation
Species: Homo sapiens, human
Tissue: brain
Disease: mixed astro-oligodendroglioma
Gender: famale,55 years
Morphology: glioblastoma
Growth Mode: fibroblastoid, adherent cells growing as monolayer
Doubling Time: 50 hours
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 7
TPOX: 9,11
vWA: 17
Cell Line Authentication Service
Culture Medium: DMEM+10%FBS+4 mM L-glutamine
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Comments: Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays Cytogenetics: human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 typical in low-grade astrocytoma-oligodendroglioma - gains of sex chromosomes (particularly Y) are very rare in tumor cells
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin + Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
產(chǎn)品類別：其它動(dòng)物細(xì)胞系
細(xì)胞形態(tài)：貼壁上皮細(xì)胞融合生長
凍存條件：90%FBS+10%DMSO
Synonyms: GPH1豚鼠心肌細(xì)胞
Background: originally described as a glioma cell line established from the tumor material of brain from a 55-year-old man with a mixed astro-oligodendroglioma (grade II/III); however, DNA fingerprinting at DSMZ showed clearly that this cell line is in reality a derivative of the human glioblastoma cell line U-343-MG; described in the literature to express neuroepithelial markers GFAP, vimentin and S-100 protein as well as pp60C-SRC, but not the neuro-specific variant pp60C-SRCN and to express neuropoietic factors CNTF, LIF and their receptors; LIF and oncostatin M induce growth inhibition and differentiation
Species: Homo sapiens, human
Tissue: brain
Disease: mixed astro-oligodendroglioma
Gender: famale,55 years
Morphology: glioblastoma
Growth Mode: fibroblastoid, adherent cells growing as monolayer
Doubling Time: 50 hours
DNA Profile: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 9,11
D5S818: 12,13
D7S820: 8,12
THO1: 7
TPOX: 9,11
vWA: 17
Cell Line Authentication Service
Culture Medium: DMEM+10%FBS+4 mM L-glutamine
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Comments: Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays Cytogenetics: human hypodiploid karyotype with 20% polypoidy - 44(40-45)<2n>XXY/XXYY, -6, +7, -10, -14, -22, der(1)del(1)(p21)t(1;9)(q42;q34), der(9)t(9;16)(p13;p11)t(1;9)(q42;q34), der(16)t(9;16)(p13;p11), der(18)t(6;18)(p12;q12.2) - gain of 7 and loss of 10 and 22 typical in low-grade astrocytoma-oligodendroglioma - gains of sex chromosomes (particularly Y) are very rare in tumor cells
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin + Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
