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平臺編號：bio-133144
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細胞信息： EPLC-272H
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規(guī)格：1×10?cells/T25培養(yǎng)瓶
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用途：STR鑒定正確
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
EPLC-272H（人肺癌鱗癌細胞）
EPLC-272H（人肺癌鱗癌細胞）
產品類別：人源細胞系
生長特性：貼壁上皮樣細胞（無尖銳輪廓）以單層形式貼壁生長
細胞形態(tài)：貼壁
凍存條件：90%FBS+10%DMSO
Synonyms: EPLC-272HEPLC-272H（人肺癌鱗癌細胞）
Background: established from a surgical lung specimen of a 57-year-old Caucasian man who underwent surgery for undifferentiated squamous cell carcinoma of the lung (without having received prior radiation or chemotherapy) in 1986
Species: human (Homo sapiens)
Tissue: lung
Disease: epidermoid lung carcinoma
Gender: N/A
Morphology: adherent epithelial-like cells (without sharp contours) growing adherently as monolayers
Growth Mode: N/A
Doubling Time: ca. 60 hours
DNA Profile: N/A
Cell Line Authentication Service
Culture Medium: RPMI-1640+20%FBS
We strongly suggest to purchase the complete medium
Cryopreservation medium: 90%FBS+10%DMSO
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 +, cytokeratin-18 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile
Species: confirmed as human with IEF of AST, LDH, MDH
Cytogenetics: human hypotriploid karyotype with 12% polyploidy - 66(59-70)<3n>XX, -Y, -4, +7, +8, -18, -19, -20, -21, +6mar, add(1)(p32), del(2)(q33), der(2;11)(q10;q10), add(3)(p25), i(5p), i(5q), add(6)(q11), add(7)(q33)x2-3, add(7)(p15), del(8)(p12-21), del(10)(p14)x1-2, add(11)(q24)x2, add(12)(p11-12), add(13)(q33), add(14)(p12), i(15q), der(17)t(3;17)(p11;p12), add(19)(q13), add(21)(p12), dmx2 - matches published karyotype
Virus ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV -
