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平臺(tái)編號(hào)：bio-136767
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國際編號(hào)：hkb-htlr4
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細(xì)胞信息： Human TLR4 Reporter HEK293 Cells (NF-κB)
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規(guī)格：3-7 x 10e6 cells
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用途：invivnogen原裝
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Human TLR4 Reporter HEK293 Cells (NF-κB)
NF-κB–SEAP reporter HEK293 cells expressing human TLR4
HEK-Blue-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.
Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue hTLR4 cells is HEK-Blue Null2 cells.
Figures
HEK-Blue-hTLR4 cells were obtained by co-transfection of the human TLR4, MD-2 and CD14 co-receptor genes, and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.
The SEAP reporter gene is placed under the control of an IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.
Stimulation with a TLR4 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue hTLR4 cells is HEK-Blue Null2 cells.
Figures
